Detailed information of TA system TA214992

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 765241..766076
• Replicon: chromosome
• Accession: NZ_CP082777
• Organism: Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K8B91_RS03675
• Protein ID: WP_001094425.1
• Coordinates: 765699..766076 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: K8B91_RS03670
• Protein ID: WP_001285575.1
• Coordinates: 765241..765609 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B91_RS03635 (761129)	761129..761809	+	681	WP_001282912.1	WYL domain-containing protein	-
K8B91_RS03640 (761960)	761960..762637	+	678	WP_001097418.1	hypothetical protein	-
K8B91_RS03645 (762643)	762643..762876	+	234	WP_001278287.1	DUF905 family protein	-
K8B91_RS03650 (762966)	762966..763784	+	819	WP_001175163.1	DUF932 domain-containing protein	-
K8B91_RS03655 (763876)	763876..764361	+	486	WP_000206654.1	antirestriction protein	-
K8B91_RS03660 (764377)	764377..764853	+	477	WP_001186771.1	RadC family protein	-
K8B91_RS03665 (764940)	764940..765161	+	222	WP_000692350.1	DUF987 domain-containing protein	-
K8B91_RS03670 (765241)	765241..765609	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B91_RS03675 (765699)	765699..766076	+	378	WP_001094425.1	TA system toxin CbtA family protein	Toxin
K8B91_RS03680 (766073)	766073..766561	+	489	WP_000761683.1	DUF5983 family protein	-
K8B91_RS03685 (766573)	766573..766770	+	198	WP_000839272.1	DUF957 domain-containing protein	-
K8B91_RS03690 (766855)	766855..767004	+	150	Protein_705	hypothetical protein	-
K8B91_RS03695 (767471)	767471..768735	+	1265	Protein_706	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papX / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI / cnf1 / hlyD / hlyB / hlyA / hlyC	687989..786068	98079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.97 Da
• Isoelectric Point: 7.3523

>T214992 WP_001094425.1 NZ_CP082777:765699-766076 [Escherichia coli O25:H1]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACSRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T214992 NZ_CP110354:3635650-3635949 [Enterobacter hormaechei]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT214992 WP_001285575.1 NZ_CP082777:765241-765609 [Escherichia coli O25:H1]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT214992 NZ_CP110354:3635960-3636325 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A454ABF5