Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3984485..3985319 Replicon chromosome
Accession NZ_CP082776
Organism Escherichia coli O4:H5 strain CU532-9

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2T3TJB4
Locus tag K8B92_RS19215 Protein ID WP_000854811.1
Coordinates 3984942..3985319 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2T3TJJ3
Locus tag K8B92_RS19210 Protein ID WP_001285590.1
Coordinates 3984485..3984853 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B92_RS19180 (3981389) 3981389..3981844 + 456 WP_000581502.1 IrmA family protein -
K8B92_RS19185 (3981945) 3981945..3982116 + 172 Protein_3738 DUF905 family protein -
K8B92_RS19190 (3982234) 3982234..3983052 + 819 WP_001234593.1 DUF932 domain-containing protein -
K8B92_RS19195 (3983144) 3983144..3983629 + 486 WP_000214310.1 antirestriction protein -
K8B92_RS19200 (3983645) 3983645..3984121 + 477 WP_001536299.1 RadC family protein -
K8B92_RS19205 (3984190) 3984190..3984411 + 222 WP_000692300.1 DUF987 domain-containing protein -
K8B92_RS19210 (3984485) 3984485..3984853 + 369 WP_001285590.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K8B92_RS19215 (3984942) 3984942..3985319 + 378 WP_000854811.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K8B92_RS19220 (3985316) 3985316..3985746 + 431 Protein_3745 DUF5983 family protein -
K8B92_RS19225 (3985763) 3985763..3985939 + 177 WP_000839288.1 DUF957 domain-containing protein -
K8B92_RS19230 (3986045) 3986045..3986275 + 231 Protein_3747 hypothetical protein -
K8B92_RS19235 (3986301) 3986301..3987521 - 1221 WP_000794251.1 capsular biosynthesis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14041.14 Da        Isoelectric Point: 7.8839

>T214987 WP_000854811.1 NZ_CP082776:3984942-3985319 [Escherichia coli O4:H5]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Download         Length: 378 bp

>T214987 NZ_CP110354:36286-36600 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13608.50 Da        Isoelectric Point: 6.6242

>AT214987 WP_001285590.1 NZ_CP082776:3984485-3984853 [Escherichia coli O4:H5]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLYSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT214987 NZ_CP110354:36600-37016 [Enterobacter hormaechei]
ATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGTCCTTTGCTGGATATTGTGTGCG
CCAGAATTAGCCATTACGAAGCTAATGGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCACCTG
CGTTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2T3TJB4

Antitoxin

Source ID Structure
AlphaFold DB A0A2T3TJJ3

References