Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3893892..3894546 Replicon chromosome
Accession NZ_CP082776
Organism Escherichia coli O4:H5 strain CU532-9

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag K8B92_RS18710 Protein ID WP_000244781.1
Coordinates 3893892..3894299 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K8B92_RS18715 Protein ID WP_000354046.1
Coordinates 3894280..3894546 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B92_RS18690 (3889849) 3889849..3891582 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
K8B92_RS18695 (3891588) 3891588..3892298 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K8B92_RS18700 (3892323) 3892323..3893219 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K8B92_RS18705 (3893331) 3893331..3893852 + 522 WP_001055874.1 flavodoxin FldB -
K8B92_RS18710 (3893892) 3893892..3894299 - 408 WP_000244781.1 protein YgfX Toxin
K8B92_RS18715 (3894280) 3894280..3894546 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K8B92_RS18720 (3894789) 3894789..3895769 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
K8B92_RS18725 (3895846) 3895846..3896505 - 660 WP_000250274.1 hemolysin III family protein -
K8B92_RS18730 (3896669) 3896669..3896980 - 312 WP_001182948.1 N(4)-acetylcytidine aminohydrolase -
K8B92_RS18735 (3897025) 3897025..3898458 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T214986 WP_000244781.1 NZ_CP082776:c3894299-3893892 [Escherichia coli O4:H5]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214986 NZ_CP110353:c5515218-5514940 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATGGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214986 WP_000354046.1 NZ_CP082776:c3894546-3894280 [Escherichia coli O4:H5]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214986 NZ_CP110353:c5514928-5514623 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References