Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2917812..2918643 Replicon chromosome
Accession NZ_CP082776
Organism Escherichia coli O4:H5 strain CU532-9

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K8B92_RS14200 Protein ID WP_000854814.1
Coordinates 2918269..2918643 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A366YXM1
Locus tag K8B92_RS14195 Protein ID WP_001518724.1
Coordinates 2917812..2918180 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B92_RS14160 (2913315) 2913315..2914048 - 734 Protein_2756 IS3 family transposase -
K8B92_RS14165 (2914105) 2914105..2914758 + 654 WP_001535423.1 hypothetical protein -
K8B92_RS14170 (2914774) 2914774..2915184 + 411 WP_000846705.1 hypothetical protein -
K8B92_RS14175 (2915405) 2915405..2916226 + 822 WP_001234710.1 DUF932 domain-containing protein -
K8B92_RS14180 (2916489) 2916489..2916962 + 474 WP_000855075.1 antirestriction protein -
K8B92_RS14185 (2916978) 2916978..2917454 + 477 WP_001186773.1 RadC family protein -
K8B92_RS14190 (2917517) 2917517..2917738 + 222 WP_000692323.1 DUF987 domain-containing protein -
K8B92_RS14195 (2917812) 2917812..2918180 + 369 WP_001518724.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K8B92_RS14200 (2918269) 2918269..2918643 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K8B92_RS14205 (2918640) 2918640..2918834 + 195 WP_000988601.1 DUF5983 family protein -
K8B92_RS14210 (2918880) 2918880..2918960 + 81 Protein_2766 hypothetical protein -
K8B92_RS14215 (2919249) 2919249..2919395 - 147 Protein_2767 transposase domain-containing protein -
K8B92_RS14220 (2919497) 2919497..2919631 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
K8B92_RS14225 (2919732) 2919732..2920061 - 330 WP_000450409.1 DUF496 family protein -
K8B92_RS14230 (2920233) 2920233..2921291 - 1059 WP_001200894.1 FUSC family protein -
K8B92_RS14235 (2921489) 2921489..2921962 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
K8B92_RS14240 (2922081) 2922081..2923247 - 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T214983 WP_000854814.1 NZ_CP082776:2918269-2918643 [Escherichia coli O4:H5]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T214983 NZ_CP110353:2807046-2807621 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13441.24 Da        Isoelectric Point: 4.7594

>AT214983 WP_001518724.1 NZ_CP082776:2917812-2918180 [Escherichia coli O4:H5]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLSSDADAYPLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLACEADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT214983 NZ_CP110353:2806579-2807049 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A366YXM1

References