Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4692455..4693255 Replicon chromosome
Accession NZ_CP082775
Organism Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag K8B89_RS23270 Protein ID WP_000342452.1
Coordinates 4692455..4692982 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag K8B89_RS23275 Protein ID WP_001277107.1
Coordinates 4692989..4693255 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B89_RS23245 (4687531) 4687531..4688298 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K8B89_RS23250 (4688295) 4688295..4689572 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
K8B89_RS23255 (4689569) 4689569..4690495 - 927 WP_234278523.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K8B89_RS23260 (4690543) 4690543..4691652 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
K8B89_RS23265 (4692075) 4692075..4692458 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
K8B89_RS23270 (4692455) 4692455..4692982 - 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
K8B89_RS23275 (4692989) 4692989..4693255 - 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
K8B89_RS23280 (4693404) 4693404..4694507 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
K8B89_RS23285 (4694778) 4694778..4695632 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
K8B89_RS23290 (4695877) 4695877..4696935 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
K8B89_RS23295 (4696928) 4696928..4697596 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T214967 WP_000342452.1 NZ_CP082775:c4692982-4692455 [Escherichia coli O25:H4]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T214967 NZ_CP110351:c142226-141945 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT214967 WP_001277107.1 NZ_CP082775:c4693255-4692989 [Escherichia coli O25:H4]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT214967 NZ_CP110351:c142450-142223 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References