Detailed information of TA system TA214964

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4160543..4161377
• Replicon: chromosome
• Accession: NZ_CP082775
• Organism: Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: K8B89_RS20620
• Protein ID: WP_000854690.1
• Coordinates: 4161000..4161377 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: K8B89_RS20615
• Protein ID: WP_001305076.1
• Coordinates: 4160543..4160911 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B89_RS20575 (4155625)	4155625..4156752	+	1128	Protein_4013	hypothetical protein	-
K8B89_RS20580 (4156828)	4156828..4157283	+	456	WP_000581502.1	IrmA family protein	-
K8B89_RS20585 (4157362)	4157362..4157595	+	234	WP_000902034.1	DUF905 family protein	-
K8B89_RS20590 (4157696)	4157696..4158514	+	819	WP_001234628.1	DUF932 domain-containing protein	-
K8B89_RS20595 (4158569)	4158569..4159054	+	486	WP_000849565.1	antirestriction protein	-
K8B89_RS20600 (4159070)	4159070..4159546	+	477	WP_001186726.1	RadC family protein	-
K8B89_RS20605 (4159609)	4159609..4159830	+	222	WP_000692329.1	DUF987 domain-containing protein	-
K8B89_RS20610 (4159849)	4159849..4160493	+	645	WP_000094916.1	hypothetical protein	-
K8B89_RS20615 (4160543)	4160543..4160911	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B89_RS20620 (4161000)	4161000..4161377	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
K8B89_RS20625 (4161374)	4161374..4161862	+	489	WP_000761699.1	DUF5983 family protein	-
K8B89_RS20630 (4161879)	4161879..4162076	+	198	WP_000839293.1	DUF957 domain-containing protein	-
K8B89_RS20635 (4162161)	4162161..4163006	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
K8B89_RS20640 (4163075)	4163075..4163470	+	396	WP_000208382.1	DUF6088 family protein	-
K8B89_RS20645 (4163463)	4163463..4164396	+	934	Protein_4027	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
K8B89_RS20650 (4164813)	4164813..4164983	+	171	Protein_4028	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4164828..4164983	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T214964 WP_000854690.1 NZ_CP082775:4161000-4161377 [Escherichia coli O25:H4]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T214964 NZ_CP110350:3139774-3140361 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT214964 WP_001305076.1 NZ_CP082775:4160543-4160911 [Escherichia coli O25:H4]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT214964 NZ_CP110350:3140424-3141278 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAGATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGACAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8