Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3035698..3036529 Replicon chromosome
Accession NZ_CP082775
Organism Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag K8B89_RS15320 Protein ID WP_000854815.1
Coordinates 3036155..3036529 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag K8B89_RS15315 Protein ID WP_001280918.1
Coordinates 3035698..3036066 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B89_RS15275 (3031289) 3031289..3031966 + 678 WP_001362823.1 hypothetical protein -
K8B89_RS15280 (3031982) 3031982..3032392 + 411 WP_000846703.1 hypothetical protein -
K8B89_RS15285 (3032613) 3032613..3033431 + 819 WP_001542275.1 DUF932 domain-containing protein -
K8B89_RS15290 (3033431) 3033431..3033676 + 246 WP_001164966.1 hypothetical protein -
K8B89_RS15295 (3033770) 3033770..3034243 + 474 WP_001542276.1 antirestriction protein -
K8B89_RS15300 (3034259) 3034259..3034735 + 477 WP_001186200.1 RadC family protein -
K8B89_RS15305 (3034798) 3034798..3035019 + 222 WP_000692345.1 DUF987 domain-containing protein -
K8B89_RS15310 (3035038) 3035038..3035682 + 645 WP_000086752.1 hypothetical protein -
K8B89_RS15315 (3035698) 3035698..3036066 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K8B89_RS15320 (3036155) 3036155..3036529 + 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K8B89_RS15325 (3036526) 3036526..3036720 + 195 WP_000988600.1 DUF5983 family protein -
K8B89_RS15330 (3036766) 3036766..3036846 + 81 Protein_2984 hypothetical protein -
K8B89_RS15335 (3037135) 3037135..3037281 - 147 Protein_2985 transposase domain-containing protein -
K8B89_RS15340 (3037383) 3037383..3037517 + 135 WP_059338447.1 EutP/PduV family microcompartment system protein -
K8B89_RS15345 (3037618) 3037618..3037947 - 330 WP_000450409.1 DUF496 family protein -
K8B89_RS15350 (3038119) 3038119..3039177 - 1059 WP_001200889.1 FUSC family protein -
K8B89_RS15355 (3039375) 3039375..3039848 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
K8B89_RS15360 (3039968) 3039968..3041134 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T214961 WP_000854815.1 NZ_CP082775:3036155-3036529 [Escherichia coli O25:H4]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T214961 NZ_CP110350:c70466-70188 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCATCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGTGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT214961 WP_001280918.1 NZ_CP082775:3035698-3036066 [Escherichia coli O25:H4]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT214961 NZ_CP110350:c70176-69871 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCCCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References