Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1229109..1229727 Replicon chromosome
Accession NZ_CP082775
Organism Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K8B89_RS05810 Protein ID WP_001291435.1
Coordinates 1229109..1229327 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K8B89_RS05815 Protein ID WP_000344800.1
Coordinates 1229353..1229727 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B89_RS05775 (1224396) 1224396..1224968 + 573 WP_000779831.1 YbaY family lipoprotein -
K8B89_RS05780 (1224999) 1224999..1225310 - 312 WP_000409908.1 MGMT family protein -
K8B89_RS05790 (1225689) 1225689..1226042 + 354 WP_234278532.1 DUF1428 family protein -
K8B89_RS05795 (1226084) 1226084..1227634 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K8B89_RS05800 (1227798) 1227798..1228268 - 471 WP_000136192.1 YlaC family protein -
K8B89_RS05805 (1228384) 1228384..1228935 - 552 WP_000102539.1 maltose O-acetyltransferase -
K8B89_RS05810 (1229109) 1229109..1229327 - 219 WP_001291435.1 HHA domain-containing protein Toxin
K8B89_RS05815 (1229353) 1229353..1229727 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K8B89_RS05820 (1230273) 1230273..1233422 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
K8B89_RS05825 (1233445) 1233445..1234638 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214946 WP_001291435.1 NZ_CP082775:c1229327-1229109 [Escherichia coli O25:H4]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T214946 NZ_CP110347:c5224506-5224228 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGTGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT214946 WP_000344800.1 NZ_CP082775:c1229727-1229353 [Escherichia coli O25:H4]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT214946 NZ_CP110347:c5224216-5223911 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAACA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References