Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 775577..776097 | Replicon | chromosome |
| Accession | NZ_CP082775 | ||
| Organism | Escherichia coli O25:H4 strain CU568-3 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | A0A061KX66 |
| Locus tag | K8B89_RS03635 | Protein ID | WP_001160964.1 |
| Coordinates | 775813..776097 (+) | Length | 95 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | V0VEX8 |
| Locus tag | K8B89_RS03630 | Protein ID | WP_000125566.1 |
| Coordinates | 775577..775810 (+) | Length | 78 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K8B89_RS03605 (770650) | 770650..770937 | + | 288 | WP_000203744.1 | ferredoxin-like protein FixX | - |
| K8B89_RS03610 (770996) | 770996..772327 | + | 1332 | WP_001183209.1 | MFS transporter | - |
| K8B89_RS03615 (772435) | 772435..772965 | + | 531 | WP_000600725.1 | glutathione-regulated potassium-efflux system oxidoreductase KefF | - |
| K8B89_RS03620 (772958) | 772958..774820 | + | 1863 | WP_000377124.1 | glutathione-regulated potassium-efflux system protein KefC | - |
| K8B89_RS03625 (775012) | 775012..775491 | + | 480 | WP_000624375.1 | type 3 dihydrofolate reductase | - |
| K8B89_RS03630 (775577) | 775577..775810 | + | 234 | WP_000125566.1 | type II toxin-antitoxin system CcdA family antitoxin | Antitoxin |
| K8B89_RS03635 (775813) | 775813..776097 | + | 285 | WP_001160964.1 | CcdB family protein | Toxin |
| K8B89_RS03640 (776124) | 776124..776972 | - | 849 | WP_000257181.1 | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH | - |
| K8B89_RS03645 (776979) | 776979..777356 | - | 378 | WP_000610901.1 | Co2+/Mg2+ efflux protein ApaG | - |
| K8B89_RS03650 (777359) | 777359..778180 | - | 822 | WP_001065365.1 | 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA | - |
| K8B89_RS03655 (778177) | 778177..779166 | - | 990 | WP_000241227.1 | 4-hydroxythreonine-4-phosphate dehydrogenase PdxA | - |
| K8B89_RS03660 (779166) | 779166..780452 | - | 1287 | WP_000800457.1 | peptidylprolyl isomerase SurA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 95 a.a. Molecular weight: 10692.54 Da Isoelectric Point: 10.3030
>T214944 WP_001160964.1 NZ_CP082775:775813-776097 [Escherichia coli O25:H4]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK
Download Length: 285 bp
>T214944 NZ_CP110347:3481367-3481945 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACACAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCTTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCG
GCCGTCGAGCAGGCCGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGTTGACCCGTCATCTCGACGC
GCCGCTGCGCGAACGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGAGCGCCGAAGCCCTGGCGGTGAAAGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGA
AGCGCAGCGCGACGCCTAG
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACACAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCTTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCG
GCCGTCGAGCAGGCCGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGTTGACCCGTCATCTCGACGC
GCCGCTGCGCGAACGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGAGCGCCGAAGCCCTGGCGGTGAAAGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGA
AGCGCAGCGCGACGCCTAG
Antitoxin
Download Length: 78 a.a. Molecular weight: 8530.53 Da Isoelectric Point: 5.8062
>AT214944 WP_000125566.1 NZ_CP082775:775577-775810 [Escherichia coli O25:H4]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
Download Length: 234 bp
>AT214944 NZ_CP110347:3480724-3481278 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTTCCCTCTTCGTCCCTGGGCCCCGCCCTGCGCCGCTGGCGCCTGCTGCACCGGGTCAAGCAGACGCA
TGCCGCCGAATTGCTCGGCGTGGCCCAATCGACCATCTCGCGCTGGGAGTCCGGTACTCAAGCGCTGCTGCTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAAGCGCCGCTGACCAGGCCCTGGCCCGCCTGGTGGAGGCCAAC
CCGCAGCCCGTGCACCTGATCTGCGACCTCACCCACCGCCTGCTGGCCTGCTCGCCGGCGCGCGCCGCGCAATTCGGCGT
GCCCCTCGGCGATCTTCTCGGCCGCTCCCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAACTACCCTCGAGG
ACCTCGGCTGGCGCGAACTGCTGGCGCCGCCGACGCTGGAGTTCGCCAGCGGGGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACACTCTCGGACGGACGCGCGGTACGCCTGGTGGAAACCCTCTGA
ATGACCGAGCCTCTTCCCTCTTCGTCCCTGGGCCCCGCCCTGCGCCGCTGGCGCCTGCTGCACCGGGTCAAGCAGACGCA
TGCCGCCGAATTGCTCGGCGTGGCCCAATCGACCATCTCGCGCTGGGAGTCCGGTACTCAAGCGCTGCTGCTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAAGCGCCGCTGACCAGGCCCTGGCCCGCCTGGTGGAGGCCAAC
CCGCAGCCCGTGCACCTGATCTGCGACCTCACCCACCGCCTGCTGGCCTGCTCGCCGGCGCGCGCCGCGCAATTCGGCGT
GCCCCTCGGCGATCTTCTCGGCCGCTCCCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAACTACCCTCGAGG
ACCTCGGCTGGCGCGAACTGCTGGCGCCGCCGACGCTGGAGTTCGCCAGCGGGGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACACTCTCGGACGGACGCGCGGTACGCCTGGTGGAAACCCTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A061KX66 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LTM9 |