Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 666411..666987 Replicon chromosome
Accession NZ_CP082775
Organism Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag K8B89_RS03100 Protein ID WP_001360091.1
Coordinates 666411..666698 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag K8B89_RS03105 Protein ID WP_000063156.1
Coordinates 666685..666987 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B89_RS03080 (662664) 662664..663944 - 1281 WP_001037380.1 DUF445 domain-containing protein -
K8B89_RS03085 (663985) 663985..665217 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
K8B89_RS03090 (665363) 665363..665458 - 96 Protein_586 dienelactone hydrolase family protein -
K8B89_RS03095 (665515) 665515..666212 + 698 Protein_587 IS1-like element IS1F family transposase -
K8B89_RS03100 (666411) 666411..666698 + 288 WP_001360091.1 BrnT family toxin Toxin
K8B89_RS03105 (666685) 666685..666987 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
K8B89_RS03110 (667021) 667021..667977 - 957 WP_001297640.1 GTPase -
K8B89_RS03115 (667988) 667988..668191 - 204 WP_000467859.1 YbdD/YjiX family protein -
K8B89_RS03120 (668241) 668241..670391 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 666003..666212 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T214943 WP_001360091.1 NZ_CP082775:666411-666698 [Escherichia coli O25:H4]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

>T214943 NZ_CP110347:1830953-1831540 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTATCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGTGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGTTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGTCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTCGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT214943 WP_000063156.1 NZ_CP082775:666685-666987 [Escherichia coli O25:H4]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT214943 NZ_CP110347:1831607-1832461 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAGCTGCTGCATGCGCG
TTATGTGGAGCAGCGTTTCGCGCCGCATGTGCACAGCAGCTTCGCCCTGGTCATCATCGAACAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGACGCCGGTTGGCTCTATCGCGGCTACTACCCGGAGATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAACCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAGACCCAGACCCGCTGGCGCGAGACGGTGCTCGCCCTGTTCCGTCGCCAT
GCCCGCCTGCCAGAGGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGACCCGCCTTCACTGGAGGAATTGGCGGCGGCGGTCAACCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References