Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 969474..970024 Replicon chromosome
Accession NZ_CP082774
Organism Escherichia coli O16:H6 strain CU37RT-2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag K8B88_RS04660 Protein ID WP_001160966.1
Coordinates 969710..970024 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag K8B88_RS04655 Protein ID WP_000125566.1
Coordinates 969474..969707 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B88_RS04630 (964547) 964547..964834 + 288 WP_000203741.1 ferredoxin-like protein FixX -
K8B88_RS04635 (964893) 964893..966224 + 1332 WP_001183207.1 MFS transporter -
K8B88_RS04640 (966332) 966332..966862 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
K8B88_RS04645 (966855) 966855..968717 + 1863 WP_021523578.1 glutathione-regulated potassium-efflux system protein KefC -
K8B88_RS04650 (968909) 968909..969388 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
K8B88_RS04655 (969474) 969474..969707 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
K8B88_RS04660 (969710) 969710..970024 + 315 WP_001160966.1 CcdB family protein Toxin
K8B88_RS04665 (970021) 970021..970869 - 849 WP_001765384.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
K8B88_RS04670 (970876) 970876..971253 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
K8B88_RS04675 (971256) 971256..972077 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
K8B88_RS04680 (972074) 972074..973063 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
K8B88_RS04685 (973063) 973063..974349 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T214920 WP_001160966.1 NZ_CP082774:969710-970024 [Escherichia coli O16:H6]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T214920 NZ_CP110344:3089247-3089546 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT214920 WP_000125566.1 NZ_CP082774:969474-969707 [Escherichia coli O16:H6]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT214920 NZ_CP110344:3089543-3089833 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References