Detailed information of TA system TA214919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 862786..863362
• Replicon: chromosome
• Accession: NZ_CP082774
• Organism: Escherichia coli O16:H6 strain CU37RT-2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: K8B88_RS04130
• Protein ID: WP_001297643.1
• Coordinates: 862786..863073 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: K8B88_RS04135
• Protein ID: WP_021538262.1
• Coordinates: 863060..863362 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B88_RS04110 (859039)	859039..860319	-	1281	WP_001037380.1	DUF445 domain-containing protein	-
K8B88_RS04115 (860360)	860360..861592	-	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
K8B88_RS04120 (861738)	861738..861833	-	96	Protein_794	dienelactone hydrolase family protein	-
K8B88_RS04125 (861890)	861890..862587	+	698	Protein_795	IS1 family transposase	-
K8B88_RS04130 (862786)	862786..863073	+	288	WP_001297643.1	BrnT family toxin	Toxin
K8B88_RS04135 (863060)	863060..863362	+	303	WP_021538262.1	BrnA antitoxin family protein	Antitoxin
K8B88_RS04140 (863396)	863396..864352	-	957	WP_001297640.1	GTPase	-
K8B88_RS04145 (864363)	864363..864566	-	204	WP_000467859.1	YbdD/YjiX family protein	-
K8B88_RS04150 (864616)	864616..866766	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T214919 WP_001297643.1 NZ_CP082774:862786-863073 [Escherichia coli O16:H6]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T214919 NZ_CP110344:2844899-2845177 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGGTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11531.00 Da
• Isoelectric Point: 9.4651

>AT214919 WP_021538262.1 NZ_CP082774:863060-863362 [Escherichia coli O16:H6]
MSMVKHKRGTSSTLDVHHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT214919 NZ_CP110344:2845189-2845494 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A829GEV9