Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 479836..480333 Replicon chromosome
Accession NZ_CP082774
Organism Escherichia coli O16:H6 strain CU37RT-2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K8B88_RS02195 Protein ID WP_001173343.1
Coordinates 480160..480333 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag K8B88_RS02190 Protein ID WP_001312331.1
Coordinates 479836..480132 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B88_RS02170 (476566) 476566..477285 - 720 WP_000611282.1 two-component system response regulator DcuR -
K8B88_RS02175 (477282) 477282..478913 - 1632 WP_001216466.1 sensor histidine kinase -
K8B88_RS02180 (479094) 479094..479324 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
K8B88_RS02185 (479336) 479336..479608 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
K8B88_RS02190 (479836) 479836..480132 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K8B88_RS02195 (480160) 480160..480333 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K8B88_RS02200 (480452) 480452..481969 - 1518 WP_000003805.1 lysine--tRNA ligase -
K8B88_RS02205 (482206) 482206..483663 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T214915 WP_001173343.1 NZ_CP082774:480160-480333 [Escherichia coli O16:H6]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T214915 NZ_CP110344:c150735-150454 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT214915 WP_001312331.1 NZ_CP082774:479836-480132 [Escherichia coli O16:H6]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT214915 NZ_CP110344:c150959-150732 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References