Detailed information of TA system TA214912

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4018768..4019566
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZRI1
• Locus tag: K8B87_RS19295
• Protein ID: WP_000854738.1
• Coordinates: 4019189..4019566 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: K8B87_RS19290
• Protein ID: WP_001285415.1
• Coordinates: 4018768..4019142 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS19255 (4014699)	4014699..4015379	+	681	WP_001282919.1	WYL domain-containing protein	-
K8B87_RS19260 (4015527)	4015527..4016204	+	678	WP_001097314.1	hypothetical protein	-
K8B87_RS19265 (4016210)	4016210..4016443	+	234	WP_001278290.1	DUF905 family protein	-
K8B87_RS19270 (4016533)	4016533..4017351	+	819	WP_021511972.1	DUF932 domain-containing protein	-
K8B87_RS19275 (4017433)	4017433..4017912	+	480	WP_000844100.1	antirestriction protein	-
K8B87_RS19280 (4017928)	4017928..4018404	+	477	WP_021511973.1	RadC family protein	-
K8B87_RS19285 (4018467)	4018467..4018688	+	222	WP_000692309.1	DUF987 domain-containing protein	-
K8B87_RS19290 (4018768)	4018768..4019142	+	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B87_RS19295 (4019189)	4019189..4019566	+	378	WP_000854738.1	TA system toxin CbtA family protein	Toxin
K8B87_RS19300 (4019563)	4019563..4020051	+	489	WP_000761688.1	DUF5983 family protein	-
K8B87_RS19305 (4020063)	4020063..4020239	+	177	WP_021573666.1	DUF957 domain-containing protein	-
K8B87_RS23655 (4020345)	4020345..4021186	+	842	Protein_3764	DUF4942 domain-containing protein	-
K8B87_RS19320 (4021528)	4021528..4021698	+	171	Protein_3765	IS110 family transposase	-
K8B87_RS19325 (4022509)	4022509..4023492	+	984	WP_123008935.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14089.12 Da
• Isoelectric Point: 8.2904

>T214912 WP_000854738.1 NZ_CP082773:4019189-4019566 [Escherichia coli O99:H6]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMVRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T214912 NZ_CP110342:4086082-4086393 [Marinobacter sp. AN1]
ATGCTATTCATCGAAACCTCAACGTTCACCAAGCTATTGCCGAACTACCTCACGGACGAGGAATATCGTGGGCTTCGAAC
CTACCTCTTGCAGAAGCCGGATGCCGGGGATCTAATCAAAGGCTCTGGTGGCGTTCGTAAAGTTCGATGGGCACCAGCGG
GTTCGGGAAAGAGCGGTGGTATCAGAGCCATTTACTACTGGAAGAAGTCTGACCACGAGATATGGATGCTCACGCTGTAC
AGTAAATCAGAGCGCGTCAGCATTCCTGGCCATACGCTCAAGCAGATAGCGGAGGCAATCAAAAATGGGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT214912 WP_001285415.1 NZ_CP082773:4018768-4019142 [Escherichia coli O99:H6]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT214912 NZ_CP110342:4086386-4086673 [Marinobacter sp. AN1]
ATGGGTGACAGGAACCTTGGGGCAGAGATTCTTGATGGCATCAATGAAATCAAGCAGTTCAAGAAGGGTAAATTGGCTCT
ACGAACCCATGAGCTATCAGAGCCCTCACCGCCTAAAGTGATCAGGCTGAAGTTGAACATGTCGCAATCTGCGTTTGCCG
GTCTTATGGGCGTGAGTGTGCGAACCCTTCAGGATTGGGAGCAGGGTCGGCGTGAACCGCAGGGGCCTGCGGTTGCGCTT
CTGCGCATTGCTGAGCAACACCCGGAAGTTTTCAATCAGCTTCACTGA

Structures

Toxin

• AlphaFold DB: A7ZRI1

Antitoxin

• AlphaFold DB: A0A067H947