Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4018768..4019566 Replicon chromosome
Accession NZ_CP082773
Organism Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)


Gene name yeeV Uniprot ID A7ZRI1
Locus tag K8B87_RS19295 Protein ID WP_000854738.1
Coordinates 4019189..4019566 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A067H947
Locus tag K8B87_RS19290 Protein ID WP_001285415.1
Coordinates 4018768..4019142 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B87_RS19255 (4014699) 4014699..4015379 + 681 WP_001282919.1 WYL domain-containing protein -
K8B87_RS19260 (4015527) 4015527..4016204 + 678 WP_001097314.1 hypothetical protein -
K8B87_RS19265 (4016210) 4016210..4016443 + 234 WP_001278290.1 DUF905 family protein -
K8B87_RS19270 (4016533) 4016533..4017351 + 819 WP_021511972.1 DUF932 domain-containing protein -
K8B87_RS19275 (4017433) 4017433..4017912 + 480 WP_000844100.1 antirestriction protein -
K8B87_RS19280 (4017928) 4017928..4018404 + 477 WP_021511973.1 RadC family protein -
K8B87_RS19285 (4018467) 4018467..4018688 + 222 WP_000692309.1 DUF987 domain-containing protein -
K8B87_RS19290 (4018768) 4018768..4019142 + 375 WP_001285415.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K8B87_RS19295 (4019189) 4019189..4019566 + 378 WP_000854738.1 TA system toxin CbtA family protein Toxin
K8B87_RS19300 (4019563) 4019563..4020051 + 489 WP_000761688.1 DUF5983 family protein -
K8B87_RS19305 (4020063) 4020063..4020239 + 177 WP_021573666.1 DUF957 domain-containing protein -
K8B87_RS23655 (4020345) 4020345..4021186 + 842 Protein_3764 DUF4942 domain-containing protein -
K8B87_RS19320 (4021528) 4021528..4021698 + 171 Protein_3765 IS110 family transposase -
K8B87_RS19325 (4022509) 4022509..4023492 + 984 WP_123008935.1 KpsF/GutQ family sugar-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14089.12 Da        Isoelectric Point: 8.2904

>T214912 WP_000854738.1 NZ_CP082773:4019189-4019566 [Escherichia coli O99:H6]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMVRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T214912 NZ_CP110342:4086082-4086393 [Marinobacter sp. AN1]
ATGCTATTCATCGAAACCTCAACGTTCACCAAGCTATTGCCGAACTACCTCACGGACGAGGAATATCGTGGGCTTCGAAC
CTACCTCTTGCAGAAGCCGGATGCCGGGGATCTAATCAAAGGCTCTGGTGGCGTTCGTAAAGTTCGATGGGCACCAGCGG
GTTCGGGAAAGAGCGGTGGTATCAGAGCCATTTACTACTGGAAGAAGTCTGACCACGAGATATGGATGCTCACGCTGTAC
AGTAAATCAGAGCGCGTCAGCATTCCTGGCCATACGCTCAAGCAGATAGCGGAGGCAATCAAAAATGGGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13663.32 Da        Isoelectric Point: 5.8640

>AT214912 WP_001285415.1 NZ_CP082773:4018768-4019142 [Escherichia coli O99:H6]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT214912 NZ_CP110342:4086386-4086673 [Marinobacter sp. AN1]
ATGGGTGACAGGAACCTTGGGGCAGAGATTCTTGATGGCATCAATGAAATCAAGCAGTTCAAGAAGGGTAAATTGGCTCT
ACGAACCCATGAGCTATCAGAGCCCTCACCGCCTAAAGTGATCAGGCTGAAGTTGAACATGTCGCAATCTGCGTTTGCCG
GTCTTATGGGCGTGAGTGTGCGAACCCTTCAGGATTGGGAGCAGGGTCGGCGTGAACCGCAGGGGCCTGCGGTTGCGCTT
CTGCGCATTGCTGAGCAACACCCGGAAGTTTTCAATCAGCTTCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZRI1

Antitoxin

Source ID Structure
AlphaFold DB A0A067H947

References