Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3749539..3750122 Replicon chromosome
Accession NZ_CP082773
Organism Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag K8B87_RS18025 Protein ID WP_000254750.1
Coordinates 3749539..3749874 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag K8B87_RS18030 Protein ID WP_000581937.1
Coordinates 3749874..3750122 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B87_RS18010 (3745425) 3745425..3746723 - 1299 WP_000036723.1 phosphopyruvate hydratase -
K8B87_RS18015 (3746811) 3746811..3748448 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
K8B87_RS18020 (3748676) 3748676..3749467 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
K8B87_RS18025 (3749539) 3749539..3749874 - 336 WP_000254750.1 endoribonuclease MazF Toxin
K8B87_RS18030 (3749874) 3749874..3750122 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
K8B87_RS18035 (3750200) 3750200..3752434 - 2235 WP_000226797.1 GTP diphosphokinase -
K8B87_RS18040 (3752482) 3752482..3753783 - 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T214910 WP_000254750.1 NZ_CP082773:c3749874-3749539 [Escherichia coli O99:H6]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T214910 NZ_CP110342:c1757382-1757104 [Marinobacter sp. AN1]
TTGATCAGATCTTTTCGGTGCAAAGAAACTCAAGGCCTGTTCGAGACGGGCAATACTCGTCGCTTCTCGGCGATAAAAAA
CGTAGCCGAACGCAAGCTGATCCTGCTGCAAGCCGCTGAAACGTTGGTTGATCTGAAATCACCGACCGGAAATCGCCTGG
AGGCCCTGAAGGGCGACCGGACTGGCCAGCATAGCATCCGGATCAATCAGCAGTGGCGGGTTTGTTTTCGATGGACCGAC
GACGGGCCGGAAGACGTGGAAATTGTGGATTACCATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT214910 WP_000581937.1 NZ_CP082773:c3750122-3749874 [Escherichia coli O99:H6]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT214910 NZ_CP110342:c1757091-1756792 [Marinobacter sp. AN1]
ATGACTCAGAATGGAATGCGCCCCATTCATCCCGGGGAAATCATCCGGGAAGACTACCTGGAGCCCACCGGGATGAGTGT
CAGCGCACTCTCCCGGGCGCTGAACGTGCCTGTAACCCGGATAAACGACGTGGTCAGGGAAAAGCGCGGGATAACCGCCG
ACACAGCCCTGCGACTGGCCCGCTATTTCGGGACCAGTGAACGCTTCTGGCTGAATCTGCAGATGGAGTATGAGCTTCGC
AAAGCCCAAATCGAGAAAGGGCCACAAGTGAATAAGGAGGTTCAGCCCAACGCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References