Detailed information of TA system TA021491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2134939..2135468
• Replicon: chromosome
• Accession: NC_007795
• Organism: Staphylococcus aureus subsp. aureus NCTC 8325

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q2FWI8
• Locus tag: SAOUHSC_02303
• Protein ID: YP_500782.1
• Coordinates: 2134939..2135301 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q2FWI7
• Locus tag: SAOUHSC_02304
• Protein ID: YP_500783.1
• Coordinates: 2135298..2135468 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAOUHSC_02298	2131929..2132699	-	771	YP_500776.1	RNA polymerase sigma factor SigB	-
SAOUHSC_02299	2132674..2133183	-	510	YP_500777.1	serine-protein kinase RsbW	-
SAOUHSC_02300	2133155..2133481	-	327	YP_500778.1	STAS domain-containing protein	-
SAOUHSC_A02189	2134741..2134974	+	234	YP_500781.1	hypothetical protein	-
SAOUHSC_02303	2134939..2135301	-	363	YP_500782.1	hypothetical protein	Toxin
SAOUHSC_02304	2135298..2135468	-	171	YP_500783.1	hypothetical protein	Antitoxin
SAOUHSC_02305	2135553..2136701	-	1149	YP_500784.1	alanine racemase	-
SAOUHSC_02306	2136767..2137126	-	360	YP_500785.1	4'-phosphopantetheinyl transferase	-
SAOUHSC_02307	2137130..2137621	-	492	YP_500786.1	hypothetical protein	-
SAOUHSC_02308	2137608..2139191	-	1584	YP_500787.1	hypothetical protein	-
SAOUHSC_02309	2139184..2139663	-	480	YP_500788.1	hypothetical protein	-
SAOUHSC_02310	2139872..2140432	-	561	YP_500789.1	potassium-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T21491 YP_500782.1 NC_007795:c2135301-2134939 [Staphylococcus aureus subsp. aureus NCTC 8325]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T21491 NC_007795:c2135301-2134939 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT21491 YP_500783.1 NC_007795:c2135468-2135298 [Staphylococcus aureus subsp. aureus NCTC 8325]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT21491 NC_007795:c2135468-2135298 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 5DLO

Antitoxin

• AlphaFold DB: Q2FWI7