Detailed information of TA system TA214908

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3619464..3620122
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: K8B87_RS17350
• Protein ID: WP_087603032.1
• Coordinates: 3619802..3620122 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: K8B87_RS17345
• Protein ID: WP_087603033.1
• Coordinates: 3619464..3619781 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS17300 (3614608)	3614608..3615432	+	825	WP_087603040.1	DUF932 domain-containing protein	-
K8B87_RS17305 (3615641)	3615641..3616351	+	711	WP_070713206.1	DeoR family transcriptional regulator	-
K8B87_RS17310 (3616377)	3616377..3616913	+	537	WP_087603039.1	DUF4339 domain-containing protein	-
K8B87_RS17315 (3616955)	3616955..3617392	+	438	WP_023301392.1	hypothetical protein	-
K8B87_RS17320 (3617459)	3617459..3617869	+	411	WP_087603038.1	hypothetical protein	-
K8B87_RS17325 (3617947)	3617947..3618183	+	237	WP_087603037.1	DUF905 domain-containing protein	-
K8B87_RS17330 (3618264)	3618264..3618722	+	459	WP_087603036.1	antirestriction protein	-
K8B87_RS17335 (3618738)	3618738..3619214	+	477	WP_087603035.1	RadC family protein	-
K8B87_RS17340 (3619223)	3619223..3619444	+	222	WP_087603034.1	DUF987 domain-containing protein	-
K8B87_RS17345 (3619464)	3619464..3619781	+	318	WP_087603033.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B87_RS17350 (3619802)	3619802..3620122	+	321	WP_087603032.1	TA system toxin CbtA family protein	Toxin
K8B87_RS17355 (3620252)	3620252..3620929	+	678	WP_032154903.1	IS66-like element accessory protein TnpA	-
K8B87_RS17360 (3620929)	3620929..3621276	+	348	WP_234257607.1	IS66 family insertion sequence element accessory protein TnpB	-
K8B87_RS17365 (3621296)	3621296..3622867	+	1572	WP_021563310.1	IS66 family transposase	-
K8B87_RS17370 (3623667)	3623667..3624644	+	978	WP_000993119.1	glutarate dioxygenase GlaH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3602404..3620122	17718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-102)

Antitoxin

• Antitoxin CbeA (5-103)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12106.14 Da
• Isoelectric Point: 7.1656

>T214908 WP_087603032.1 NZ_CP082773:3619802-3620122 [Escherichia coli O99:H6]
MKTLPATTLWAAKPCPSPVIVWQILLSRLLDQHYGLTLNDTPFSDETVIKEHIDAGISLADAVNFLVEKYELVRIDRKGL
SWQEQSPFITAVDILKARRAMREMRI

Nucleotide

• Length: 321 bp

>T214908 NZ_CP110339:3916664-3916918 [Brucella sp. JSBI001]
ATGGAACAGATCACTTACTCCCGCGAAGCAACCAAGACCTTGATCAAGATGCCAGCCAACACGGCGAAGCTGATCCGGTC
GAAGATCGAACAATATGCCGCCGATCCGGCGAGCCTTGCGAACAACGTTAAGGCGCTCAAGGGCGGCGAAGGGTTGCTCC
GCCTTCGAGTTGGCGACTGGCGGGTTATCTTCACAGAAGATGGCCGCATTATCGAAGTAATCAGGATAGCGCCGCGTGGC
GGCGCATACGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11830.44 Da
• Isoelectric Point: 6.6236

>AT214908 WP_087603033.1 NZ_CP082773:3619464-3619781 [Escherichia coli O99:H6]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLNLDVVFPHFISQIESMLTTGEQNPRHAHCVTLYH
NGFTCEVDTLGSCGYVYIAIYPTLR

Nucleotide

• Length: 318 bp

>AT214908 NZ_CP110339:3916930-3917301 [Brucella sp. JSBI001]
ATGAACGTTCAAATCATCAAGACGCCGCAGGGGGAGGAAATGGCGGTTCTCCCCAAGGCTGATTACGATAAGCTTCTTGA
AGCTTTTGAGGATCGCGAAGACATTGCAGCCGCCCGCACCTTCCGCGAAAAGCTGGCCGCCGGTGAAGAAGAATTGATCC
CGGCGGAATTCGTCAATCGCATGATCGACGGCGAGAACAAAATCAAGGTCTGGCGCGACTATCGCGGTATGAGCGCGAAG
GCACTCGCCGAGGCTGCCGGTATTAGTGCCGCCTACTTGTCGCAGATTGAAAAGGGTGCCCGCGAAGGCTCTCTCGACGC
GATGAAGAAGATTGCCGGTGCCCTCTGTGTGACGATTGATGAATTAGTATGA