Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2002959..2003757 Replicon chromosome
Accession NZ_CP082773
Organism Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag K8B87_RS09470 Protein ID WP_000854730.1
Coordinates 2003380..2003757 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P7R0L9
Locus tag K8B87_RS09465 Protein ID WP_001285482.1
Coordinates 2002959..2003333 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B87_RS09440 (1999334) 1999334..2000905 - 1572 WP_021563310.1 IS66 family transposase -
K8B87_RS09445 (2000925) 2000925..2001272 - 348 WP_000624618.1 IS66 family insertion sequence element accessory protein TnpB -
K8B87_RS09450 (2001272) 2001272..2001949 - 678 WP_032154903.1 IS66-like element accessory protein TnpA -
K8B87_RS09455 (2002025) 2002025..2002246 + 222 WP_000692315.1 DUF987 domain-containing protein -
K8B87_RS09460 (2002265) 2002265..2002909 + 645 WP_087603051.1 antitoxin of toxin-antitoxin stability system -
K8B87_RS09465 (2002959) 2002959..2003333 + 375 WP_001285482.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K8B87_RS09470 (2003380) 2003380..2003757 + 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
K8B87_RS09475 (2003754) 2003754..2004257 + 504 WP_087602440.1 DUF5983 family protein -
K8B87_RS09480 (2004254) 2004254..2004451 + 198 WP_042022639.1 DUF957 domain-containing protein -
K8B87_RS09485 (2004548) 2004548..2005391 + 844 Protein_1835 DUF4942 domain-containing protein -
K8B87_RS09495 (2005862) 2005862..2006800 + 939 WP_087602441.1 glyoxylate/hydroxypyruvate reductase GhrA -
K8B87_RS09500 (2006855) 2006855..2007592 + 738 WP_000283664.1 zinc-binding phosphatase -
K8B87_RS09505 (2007616) 2007616..2008170 + 555 WP_001764415.1 molecular chaperone YcdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T214896 WP_000854730.1 NZ_CP082773:2003380-2003757 [Escherichia coli O99:H6]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T214896 NZ_CP110313:c5121618-5121505 [Xanthomonas phaseoli pv. phaseoli]
TTGAAGGGCGAGCGGCGCGGCCAGTACCGCATCCGGATCAACGATCGATGGCGCATCTGCGTCCGATGGATGGAAGGCGA
TGTGGTCCAGGTCGAAATCATCGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13791.44 Da        Isoelectric Point: 4.7511

>AT214896 WP_001285482.1 NZ_CP082773:2002959-2003333 [Escherichia coli O99:H6]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT214896 NZ_CP110313:c5121487-5121197 [Xanthomonas phaseoli pv. phaseoli]
ATGACGGTCCTGCCCAATATCCACCCTGGCGCAATTCTGCTTGAGGAGTTTCTCCAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAAAAAAAGCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGAACCTTCGAGCGCTTCTGGCTTGGCCTGCAGGCCGACGACGAGCTGGAGCAGGCG
CATCGCGCGCTGGGCGACTTGCCGGCGCGGATCAAGCGGCTGGCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P7R0L9

References