Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1386509..1387128 Replicon chromosome
Accession NZ_CP082773
Organism Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K8B87_RS06525 Protein ID WP_001291435.1
Coordinates 1386509..1386727 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K8B87_RS06530 Protein ID WP_000344800.1
Coordinates 1386754..1387128 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B87_RS06490 (1381797) 1381797..1382369 + 573 WP_000779831.1 YbaY family lipoprotein -
K8B87_RS06495 (1382400) 1382400..1382711 - 312 WP_000409908.1 MGMT family protein -
K8B87_RS06505 (1383090) 1383090..1383443 + 354 WP_000878140.1 DUF1428 domain-containing protein -
K8B87_RS06510 (1383485) 1383485..1385035 - 1551 WP_001524176.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K8B87_RS06515 (1385199) 1385199..1385669 - 471 WP_000136192.1 YlaC family protein -
K8B87_RS06520 (1385785) 1385785..1386336 - 552 WP_000102539.1 maltose O-acetyltransferase -
K8B87_RS06525 (1386509) 1386509..1386727 - 219 WP_001291435.1 HHA domain-containing protein Toxin
K8B87_RS06530 (1386754) 1386754..1387128 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K8B87_RS06535 (1387673) 1387673..1390822 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
K8B87_RS06540 (1390845) 1390845..1392038 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214895 WP_001291435.1 NZ_CP082773:c1386727-1386509 [Escherichia coli O99:H6]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T214895 NZ_CP110313:c4575057-4574905 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGTCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAGTGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT214895 WP_000344800.1 NZ_CP082773:c1387128-1386754 [Escherichia coli O99:H6]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT214895 NZ_CP110313:c4574830-4574696 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References