Detailed information of TA system TA214894

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1072346..1073144
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K8B87_RS05070
• Protein ID: WP_234257760.1
• Coordinates: 1072346..1072723 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: K8B87_RS05075
• Protein ID: WP_001285482.1
• Coordinates: 1072770..1073144 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS05045 (1067602)	1067602..1069006	+	1405	Protein_966	IS66 family transposase	-
K8B87_RS05050 (1069260)	1069260..1070393	-	1134	WP_000555401.1	IS110-like element ISEc45 family transposase	-
K8B87_RS05055 (1070454)	1070454..1070642	+	189	Protein_968	IS66 family transposase	-
K8B87_RS05060 (1070790)	1070790..1071778	+	989	Protein_969	IS630 family transposase	-
K8B87_RS05065 (1071857)	1071857..1072349	-	493	Protein_970	DUF5983 family protein	-
K8B87_RS05070 (1072346)	1072346..1072723	-	378	WP_234257760.1	TA system toxin CbtA family protein	Toxin
K8B87_RS05075 (1072770)	1072770..1073144	-	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B87_RS05080 (1073194)	1073194..1073838	-	645	WP_087603051.1	antitoxin of toxin-antitoxin stability system	-
K8B87_RS05085 (1073857)	1073857..1074078	-	222	WP_000692315.1	DUF987 domain-containing protein	-
K8B87_RS05090 (1074141)	1074141..1074617	-	477	WP_001439314.1	RadC family protein	-
K8B87_RS05095 (1074633)	1074633..1075103	-	471	WP_016232931.1	antirestriction protein	-
K8B87_RS05100 (1075197)	1075197..1075442	-	246	WP_001164966.1	hypothetical protein	-
K8B87_RS05105 (1075442)	1075442..1076263	-	822	WP_021561928.1	DUF932 domain-containing protein	-
K8B87_RS05110 (1076363)	1076363..1076596	-	234	WP_001213776.1	DUF905 family protein	-
K8B87_RS05115 (1076714)	1076714..1077166	-	453	WP_000682723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14224.26 Da
• Isoelectric Point: 7.2923

>T214894 WP_234257760.1 NZ_CP082773:c1072723-1072346 [Escherichia coli O99:H6]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMIRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T214894 NZ_CP110313:c4574830-4574696 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT214894 WP_001285482.1 NZ_CP082773:c1073144-1072770 [Escherichia coli O99:H6]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT214894 NZ_CP110313:c4575057-4574905 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGTCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAGTGCAGCGACGGTAAGTGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0P7R0L9