Detailed information of TA system TA214893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 878192..878742
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: K8B87_RS04155
• Protein ID: WP_021513468.1
• Coordinates: 878428..878742 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: K8B87_RS04150
• Protein ID: WP_000125566.1
• Coordinates: 878192..878425 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS04125 (873265)	873265..873552	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
K8B87_RS04130 (873611)	873611..874942	+	1332	WP_001183207.1	MFS transporter	-
K8B87_RS04135 (875050)	875050..875580	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
K8B87_RS04140 (875573)	875573..877435	+	1863	WP_087602313.1	glutathione-regulated potassium-efflux system protein KefC	-
K8B87_RS04145 (877627)	877627..878106	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
K8B87_RS04150 (878192)	878192..878425	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
K8B87_RS04155 (878428)	878428..878742	+	315	WP_021513468.1	CcdB family protein	Toxin
K8B87_RS04160 (878739)	878739..879587	-	849	WP_000257182.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
K8B87_RS04165 (879594)	879594..879971	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
K8B87_RS04170 (879974)	879974..880795	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
K8B87_RS04175 (880792)	880792..881781	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
K8B87_RS04180 (881781)	881781..883067	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11697.69 Da
• Isoelectric Point: 9.0851

>T214893 WP_021513468.1 NZ_CP082773:878428-878742 [Escherichia coli O99:H6]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T214893 NZ_CP110312:c11527-11111 [Xanthomonas phaseoli pv. phaseoli]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT214893 WP_000125566.1 NZ_CP082773:878192-878425 [Escherichia coli O99:H6]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT214893 NZ_CP110312:c11778-11524 [Xanthomonas phaseoli pv. phaseoli]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCGTCCCG
CCGAGGCTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9