Detailed information of TA system TA214888

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 705512..706213
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: K8B87_RS03325
• Protein ID: WP_087602994.1
• Coordinates: 705878..706213 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K8B87_RS03320
• Protein ID: WP_032149459.1
• Coordinates: 705512..705847 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS03295 (701459)	701459..701677	-	219	WP_001683511.1	AlpA family phage regulatory protein	-
K8B87_RS03300 (701795)	701795..702409	-	615	WP_001683512.1	inovirus Gp2 family protein	-
K8B87_RS03305 (703002)	703002..703955	+	954	WP_087602991.1	hypothetical protein	-
K8B87_RS03310 (704191)	704191..705009	+	819	WP_087602992.1	DUF932 domain-containing protein	-
K8B87_RS03315 (705039)	705039..705512	+	474	WP_087602993.1	DNA repair protein RadC	-
K8B87_RS03320 (705512)	705512..705847	+	336	WP_032149459.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B87_RS03325 (705878)	705878..706213	+	336	WP_087602994.1	TA system toxin CbtA family protein	Toxin
K8B87_RS03330 (706344)	706344..707177	+	834	WP_087602995.1	DUF4942 domain-containing protein	-
K8B87_RS03335 (707254)	707254..707502	+	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
K8B87_RS03340 (707506)	707506..707781	+	276	WP_001683517.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K8B87_RS03345 (707895)	707895..708692	+	798	WP_001552326.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	688551..721684	33133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12810.86 Da
• Isoelectric Point: 8.9372

>T214888 WP_087602994.1 NZ_CP082773:705878-706213 [Escherichia coli O99:H6]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGIPLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTP

Nucleotide

• Length: 336 bp

>T214888 NZ_CP110308:50007-50423 [Xanthomonas phaseoli pv. phaseoli]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12554.52 Da
• Isoelectric Point: 7.7723

>AT214888 WP_032149459.1 NZ_CP082773:705512-705847 [Escherichia coli O99:H6]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELTPCQQR
TVTLYAKGLTCEADTLGSSGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT214888 NZ_CP110308:49756-50010 [Xanthomonas phaseoli pv. phaseoli]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCGTCCCG
CCGAGGCTTGAATGA