Detailed information of TA system TA214887

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 494470..495305
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K8B87_RS02260
• Protein ID: WP_000854743.1
• Coordinates: 494470..494847 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K8B87_RS02265
• Protein ID: WP_001285298.1
• Coordinates: 494937..495305 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS02235 (490563)	490563..492101	-	1539	WP_087602962.1	lysine decarboxylation/transport transcriptional activator CadC	-
K8B87_RS23625 (492850)	492850..493691	-	842	Protein_420	DUF4942 domain-containing protein	-
K8B87_RS02250 (493797)	493797..493973	-	177	WP_021573666.1	DUF957 domain-containing protein	-
K8B87_RS02255 (493985)	493985..494473	-	489	WP_000761693.1	DUF5983 family protein	-
K8B87_RS02260 (494470)	494470..494847	-	378	WP_000854743.1	TA system toxin CbtA family protein	Toxin
K8B87_RS02265 (494937)	494937..495305	-	369	WP_001285298.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B87_RS02270 (495355)	495355..495999	-	645	WP_000086754.1	hypothetical protein	-
K8B87_RS02275 (496014)	496014..496235	-	222	WP_000692318.1	DUF987 domain-containing protein	-
K8B87_RS02280 (496304)	496304..496780	-	477	WP_087603071.1	RadC family protein	-
K8B87_RS02285 (496796)	496796..497281	-	486	WP_000206660.1	antirestriction protein	-
K8B87_RS02290 (497373)	497373..498194	-	822	WP_016237395.1	DUF932 domain-containing protein	-
K8B87_RS02295 (498284)	498284..498517	-	234	WP_032165978.1	DUF905 family protein	-
K8B87_RS02300 (498523)	498523..499200	-	678	WP_234257695.1	hypothetical protein	-
K8B87_RS02305 (499320)	499320..500204	-	885	WP_032165981.1	YfjP family GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papI / papB / papA / papH / papC / papD / papJ / papK / papE / papF / sfaX	483074..541262	58188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14172.08 Da
• Isoelectric Point: 6.8519

>T214887 WP_000854743.1 NZ_CP082773:c494847-494470 [Escherichia coli O99:H6]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDSVNFLVEKYALVRTDQPGF
GACTYSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T214887 NZ_CP110307:48065-48481 [Xanthomonas phaseoli pv. phaseoli]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13710.44 Da
• Isoelectric Point: 5.8799

>AT214887 WP_001285298.1 NZ_CP082773:c495305-494937 [Escherichia coli O99:H6]
MSDSRHETNYPDDHNDRPWWGLPCTVIPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKN

Nucleotide

• Length: 369 bp

>AT214887 NZ_CP110307:47814-48068 [Xanthomonas phaseoli pv. phaseoli]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCGTCCCG
CCGAGGCTTGAATGA