Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1440718..1441336 Replicon chromosome
Accession NZ_CP082772
Organism Escherichia coli O2:H1 strain HM670

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K8B93_RS06725 Protein ID WP_001291435.1
Coordinates 1440718..1440936 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K8B93_RS06730 Protein ID WP_000344800.1
Coordinates 1440962..1441336 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B93_RS06690 (1436006) 1436006..1436578 + 573 WP_000779831.1 YbaY family lipoprotein -
K8B93_RS06695 (1436609) 1436609..1436920 - 312 WP_000409908.1 MGMT family protein -
K8B93_RS06705 (1437299) 1437299..1437652 + 354 WP_000878140.1 DUF1428 domain-containing protein -
K8B93_RS06710 (1437694) 1437694..1439244 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K8B93_RS06715 (1439408) 1439408..1439878 - 471 WP_001304825.1 YlaC family protein -
K8B93_RS06720 (1439994) 1439994..1440545 - 552 WP_000102539.1 maltose O-acetyltransferase -
K8B93_RS06725 (1440718) 1440718..1440936 - 219 WP_001291435.1 HHA domain-containing protein Toxin
K8B93_RS06730 (1440962) 1440962..1441336 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K8B93_RS06735 (1441881) 1441881..1445030 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
K8B93_RS06740 (1445053) 1445053..1446246 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214863 WP_001291435.1 NZ_CP082772:c1440936-1440718 [Escherichia coli O2:H1]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T214863 NZ_CP110294:504568-504909 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT214863 WP_000344800.1 NZ_CP082772:c1441336-1440962 [Escherichia coli O2:H1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT214863 NZ_CP110294:504338-504568 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References