Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 946749..947299 Replicon chromosome
Accession NZ_CP082772
Organism Escherichia coli O2:H1 strain HM670

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag K8B93_RS04415 Protein ID WP_001160966.1
Coordinates 946985..947299 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag K8B93_RS04410 Protein ID WP_000125566.1
Coordinates 946749..946982 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B93_RS04385 (941822) 941822..942109 + 288 WP_000203741.1 ferredoxin-like protein FixX -
K8B93_RS04390 (942168) 942168..943499 + 1332 WP_001183207.1 MFS transporter -
K8B93_RS04395 (943607) 943607..944137 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
K8B93_RS04400 (944130) 944130..945992 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
K8B93_RS04405 (946184) 946184..946663 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
K8B93_RS04410 (946749) 946749..946982 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
K8B93_RS04415 (946985) 946985..947299 + 315 WP_001160966.1 CcdB family protein Toxin
K8B93_RS04420 (947296) 947296..948144 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
K8B93_RS04425 (948151) 948151..948528 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
K8B93_RS04430 (948531) 948531..949352 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
K8B93_RS04435 (949349) 949349..950338 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
K8B93_RS04440 (950338) 950338..951624 - 1287 WP_171274433.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T214861 WP_001160966.1 NZ_CP082772:946985-947299 [Escherichia coli O2:H1]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T214861 NZ_CP110290:c69132-68791 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT214861 WP_000125566.1 NZ_CP082772:946749-946982 [Escherichia coli O2:H1]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT214861 NZ_CP110290:c69362-69132 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References