Detailed information of TA system TA214856

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 494134..494969
• Replicon: chromosome
• Accession: NZ_CP082772
• Organism: Escherichia coli O2:H1 strain HM670

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VR61
• Locus tag: K8B93_RS02255
• Protein ID: WP_000854747.1
• Coordinates: 494134..494511 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K8B93_RS02260
• Protein ID: WP_024182258.1
• Coordinates: 494601..494969 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B93_RS02235 (490226)	490226..491764	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
K8B93_RS02240 (492501)	492501..493355	-	855	WP_001588930.1	DUF4942 domain-containing protein	-
K8B93_RS02245 (493440)	493440..493637	-	198	WP_000839291.1	DUF957 domain-containing protein	-
K8B93_RS02250 (493649)	493649..494137	-	489	WP_000761667.1	DUF5983 family protein	-
K8B93_RS02255 (494134)	494134..494511	-	378	WP_000854747.1	TA system toxin CbtA family protein	Toxin
K8B93_RS02260 (494601)	494601..494969	-	369	WP_024182258.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B93_RS02265 (495048)	495048..495269	-	222	WP_000692312.1	DUF987 domain-containing protein	-
K8B93_RS02270 (495338)	495338..495814	-	477	WP_001186756.1	RadC family protein	-
K8B93_RS02275 (495829)	495829..496314	-	486	WP_021528701.1	antirestriction protein	-
K8B93_RS02280 (496405)	496405..497223	-	819	WP_001175140.1	DUF932 domain-containing protein	-
K8B93_RS02285 (497313)	497313..497489	-	177	WP_015674954.1	DUF905 family protein	-
K8B93_RS02290 (497514)	497514..498191	-	678	WP_001097307.1	hypothetical protein	-
K8B93_RS02295 (498339)	498339..499019	-	681	WP_001282927.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14095.03 Da
• Isoelectric Point: 6.8524

>T214856 WP_000854747.1 NZ_CP082772:c494511-494134 [Escherichia coli O2:H1]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNDITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T214856 NZ_CP110289:2729736-2729885 [Enterococcus faecalis]
ATGCGTATGCACGTATTTCCGAAATTTAGAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGG
GTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13497.32 Da
• Isoelectric Point: 6.7344

>AT214856 WP_024182258.1 NZ_CP082772:c494969-494601 [Escherichia coli O2:H1]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLGQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT214856 NZ_CP110289:c2730002-2729818 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A0H2VFZ1