214855

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 482413..482910 Replicon chromosome
Accession NZ_CP082772
Organism Escherichia coli O2:H1 strain HM670

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K8B93_RS02210 Protein ID WP_001173343.1
Coordinates 482737..482910 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag K8B93_RS02205 Protein ID WP_001312331.1
Coordinates 482413..482709 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B93_RS02185 (479142) 479142..479861 - 720 WP_000611291.1 two-component system response regulator DcuR -
K8B93_RS02190 (479858) 479858..481489 - 1632 WP_001216466.1 sensor histidine kinase -
K8B93_RS02195 (481670) 481670..481900 + 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
K8B93_RS02200 (481912) 481912..482184 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
K8B93_RS02205 (482413) 482413..482709 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K8B93_RS02210 (482737) 482737..482910 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K8B93_RS02215 (483029) 483029..484546 - 1518 WP_000003806.1 lysine--tRNA ligase -
K8B93_RS02220 (484783) 484783..486240 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T214855 WP_001173343.1 NZ_CP082772:482737-482910 [Escherichia coli O2:H1]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T214855 NZ_CP110289:c2724296-2723955 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT214855 WP_001312331.1 NZ_CP082772:482413-482709 [Escherichia coli O2:H1]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT214855 NZ_CP110289:c2724526-2724296 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References