Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3690321..3690904 Replicon chromosome
Accession NZ_CP082771
Organism Escherichia coli LF82

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag K8B90_RS17940 Protein ID WP_000254750.1
Coordinates 3690321..3690656 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag K8B90_RS17945 Protein ID WP_000581937.1
Coordinates 3690656..3690904 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B90_RS17925 (3686207) 3686207..3687505 - 1299 WP_000036723.1 phosphopyruvate hydratase -
K8B90_RS17930 (3687593) 3687593..3689230 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
K8B90_RS17935 (3689458) 3689458..3690249 - 792 WP_001071670.1 nucleoside triphosphate pyrophosphohydrolase -
K8B90_RS17940 (3690321) 3690321..3690656 - 336 WP_000254750.1 endoribonuclease MazF Toxin
K8B90_RS17945 (3690656) 3690656..3690904 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
K8B90_RS17950 (3690982) 3690982..3693216 - 2235 WP_000226815.1 GTP pyrophosphokinase -
K8B90_RS17955 (3693264) 3693264..3694565 - 1302 WP_000046827.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T214849 WP_000254750.1 NZ_CP082771:c3690656-3690321 [Escherichia coli LF82]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T214849 NZ_CP110287:1414956-1415306 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT214849 WP_000581937.1 NZ_CP082771:c3690904-3690656 [Escherichia coli LF82]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT214849 NZ_CP110287:1414664-1414951 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References