Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2857929..2858519 Replicon chromosome
Accession NZ_CP082771
Organism Escherichia coli LF82

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag K8B90_RS14005 Protein ID WP_000373724.1
Coordinates 2857929..2858261 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag K8B90_RS14010 Protein ID WP_000288812.1
Coordinates 2858262..2858519 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B90_RS13985 (2853392) 2853392..2854654 + 1263 Protein_2720 integrase arm-type DNA-binding domain-containing protein -
K8B90_RS13990 (2854793) 2854793..2855251 - 459 WP_000526101.1 IS200/IS605-like element IS200C family transposase -
K8B90_RS13995 (2856203) 2856203..2857081 - 879 WP_000511773.1 integrase domain-containing protein -
K8B90_RS14000 (2857460) 2857460..2857882 + 423 WP_001164104.1 hypothetical protein -
K8B90_RS14005 (2857929) 2857929..2858261 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K8B90_RS14010 (2858262) 2858262..2858519 - 258 WP_000288812.1 antitoxin Antitoxin
K8B90_RS14015 (2858617) 2858617..2858808 + 192 WP_077561361.1 hypothetical protein -
K8B90_RS14020 (2858867) 2858867..2859073 - 207 WP_000983730.1 helix-turn-helix transcriptional regulator -
K8B90_RS14025 (2859070) 2859070..2859537 - 468 WP_000201267.1 hypothetical protein -
K8B90_RS14030 (2859770) 2859770..2860906 - 1137 WP_000420971.1 ISAs1-like element ISEc1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2851911..2859073 7162
- inside IScluster/Tn - - 2854793..2860906 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T214847 WP_000373724.1 NZ_CP082771:c2858261-2857929 [Escherichia coli LF82]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T214847 NZ_CP110285:240648-240998 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT214847 WP_000288812.1 NZ_CP082771:c2858519-2858262 [Escherichia coli LF82]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT214847 NZ_CP110285:240356-240643 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References