Detailed information of TA system TA214825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 134..5522
• Replicon: plasmid pKP12_6
• Accession: NZ_CP082770
• Organism: Klebsiella pneumoniae strain 12

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: FTO26_RS29500
• Protein ID: WP_004197762.1
• Coordinates: 134..400 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: FTO26_RS29495
• Protein ID: WP_000879771.1
• Coordinates: 5522..150 (+)
• Length: -1790.3333333333 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTO26_RS29500 (FTO26_029500)	134..400	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FTO26_RS29505 (FTO26_029505)	470..820	-	351	WP_004197756.1	hypothetical protein	-
FTO26_RS29510 (FTO26_029510)	886..1431	-	546	WP_023317533.1	hypothetical protein	-
FTO26_RS29515 (FTO26_029515)	1457..1783	-	327	WP_050516389.1	hypothetical protein	-
FTO26_RS29520 (FTO26_029520)	2939..3115	+	177	WP_004197757.1	hypothetical protein	-
FTO26_RS29525 (FTO26_029525)	3105..3449	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
FTO26_RS29935	3658..4092	+	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
FTO26_RS29535 (FTO26_029535)	4127..4636	+	510	WP_032433972.1	MbeB family mobilization protein	-
FTO26_RS29540 (FTO26_029540)	4643..4855	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T214825 WP_004197762.1 NZ_CP082770:134-400 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T214825 NZ_CP110265:350625-350972 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: -1790.3333333333 a.a.
• Molecular weight: 10873.64 Da
• Isoelectric Point: 10.1464

>AT214825 WP_000879771.1 NZ_CP082770:5522-150 [Klebsiella pneumoniae]

Nucleotide

• Length: -5371 bp

>AT214825 NZ_CP110265:350972-351271 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5