Detailed information of TA system TA214824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5804..6384
• Replicon: plasmid pKP12_5
• Accession: NZ_CP082769
• Organism: Klebsiella pneumoniae strain 12

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: FTO26_RS29465
• Protein ID: WP_071177730.1
• Coordinates: 6070..6384 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: FTO26_RS29460
• Protein ID: WP_000093040.1
• Coordinates: 5804..6082 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTO26_RS29440 (FTO26_029440)	2245..3732	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
FTO26_RS29445 (FTO26_029445)	4377..4622	+	246	WP_032440458.1	hypothetical protein	-
FTO26_RS29450 (FTO26_029450)	4896..5267	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
FTO26_RS29850	5264..5629	+	366	WP_072354022.1	TonB family protein	-
FTO26_RS29460 (FTO26_029460)	5804..6082	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
FTO26_RS29465 (FTO26_029465)	6070..6384	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FTO26_RS29470 (FTO26_029470)	6548..6976	+	429	WP_001140599.1	hypothetical protein	-
FTO26_RS29475 (FTO26_029475)	7002..7181	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
FTO26_RS29480 (FTO26_029480)	7208..7738	-	531	WP_071177729.1	hypothetical protein	-
FTO26_RS29485 (FTO26_029485)	7745..8476	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
FTO26_RS29490 (FTO26_029490)	8476..10440	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T214824 WP_071177730.1 NZ_CP082769:6070-6384 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T214824 NZ_CP110265:324656-325024 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT214824 WP_000093040.1 NZ_CP082769:5804-6082 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT214824 NZ_CP110265:324438-324659 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGCTCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1