Detailed information of TA system TA214818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 125356..126026
• Replicon: plasmid pKP12-vir
• Accession: NZ_CP082766
• Organism: Klebsiella pneumoniae strain 12

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: FTO26_RS27715
• Protein ID: WP_004213072.1
• Coordinates: 125356..125799 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: FTO26_RS27720
• Protein ID: WP_004213073.1
• Coordinates: 125796..126026 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTO26_RS27680 (FTO26_027680)	120767..121042	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
FTO26_RS27685 (FTO26_027685)	121105..121596	+	492	WP_011251327.1	DM13 domain-containing protein	-
FTO26_RS27690 (FTO26_027690)	121645..122565	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
FTO26_RS27695 (FTO26_027695)	122656..123059	+	404	Protein_142	GAF domain-containing protein	-
FTO26_RS27700 (FTO26_027700)	123577..124212	-	636	Protein_143	mucoid phenotype regulator RmpA2	-
FTO26_RS27705 (FTO26_027705)	124629..124933	+	305	Protein_144	transposase	-
FTO26_RS29790	124956..125207	-	252	WP_186987481.1	hypothetical protein	-
FTO26_RS27715 (FTO26_027715)	125356..125799	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
FTO26_RS27720 (FTO26_027720)	125796..126026	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FTO26_RS27725 (FTO26_027725)	126634..127767	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
FTO26_RS27730 (FTO26_027730)	127783..128076	+	294	WP_004213076.1	hypothetical protein	-
FTO26_RS27735 (FTO26_027735)	128066..128272	-	207	WP_004213077.1	hypothetical protein	-
FTO26_RS27740 (FTO26_027740)	128624..128914	+	291	WP_004213078.1	hypothetical protein	-
FTO26_RS27745 (FTO26_027745)	128904..129803	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..199279	199279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T214818 WP_004213072.1 NZ_CP082766:c125799-125356 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T214818 NZ_CP110261:81007-81405 [Escherichia albertii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT214818 WP_004213073.1 NZ_CP082766:c126026-125796 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT214818 NZ_CP110261:80780-81007 [Escherichia albertii]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620