Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 124157..124827 Replicon plasmid pKP36-vir
Accession NZ_CP082760
Organism Klebsiella pneumoniae strain 36

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag FTO14_RS27715 Protein ID WP_004213072.1
Coordinates 124157..124600 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag FTO14_RS27720 Protein ID WP_004213073.1
Coordinates 124597..124827 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTO14_RS27680 (FTO14_027680) 119568..119843 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
FTO14_RS27685 (FTO14_027685) 119906..120397 + 492 WP_011251327.1 DM13 domain-containing protein -
FTO14_RS27690 (FTO14_027690) 120446..121366 + 921 WP_004213934.1 DUF1471 domain-containing protein -
FTO14_RS27695 (FTO14_027695) 121457..121860 + 404 Protein_140 GAF domain-containing protein -
FTO14_RS27700 (FTO14_027700) 122378..123013 - 636 Protein_141 mucoid phenotype regulator RmpA2 -
FTO14_RS27705 (FTO14_027705) 123430..123734 + 305 Protein_142 transposase -
FTO14_RS29785 123757..124008 - 252 WP_186987481.1 hypothetical protein -
FTO14_RS27715 (FTO14_027715) 124157..124600 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
FTO14_RS27720 (FTO14_027720) 124597..124827 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FTO14_RS27725 (FTO14_027725) 125435..126568 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
FTO14_RS27730 (FTO14_027730) 126584..126877 + 294 WP_004213076.1 hypothetical protein -
FTO14_RS27735 (FTO14_027735) 126867..127073 - 207 WP_004213077.1 hypothetical protein -
FTO14_RS27740 (FTO14_027740) 127425..127715 + 291 WP_004213078.1 hypothetical protein -
FTO14_RS27745 (FTO14_027745) 127705..128604 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..197408 197408


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T214791 WP_004213072.1 NZ_CP082760:c124600-124157 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T214791 NZ_CP110258:166254-166406 [Escherichia albertii]
ATGCCGCAGAAATATAGATTGCTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGGGATTC
ACTGTGTGAATTGCATATTAAACAGGGGAGTTATGAGCTGGCGGCGTTTTTAGCCTGCAATTTGAAAGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT214791 WP_004213073.1 NZ_CP082760:c124827-124597 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT214791 NZ_CP110258:c166197-166149 [Escherichia albertii]
GCATAGAGGGGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q6U619


Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References