Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5399246..5399871 Replicon chromosome
Accession NZ_CP082759
Organism Klebsiella pneumoniae strain 36

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FTO14_RS26635 Protein ID WP_002882817.1
Coordinates 5399246..5399629 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FTO14_RS26640 Protein ID WP_004150355.1
Coordinates 5399629..5399871 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTO14_RS26620 (FTO14_026620) 5396612..5397514 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FTO14_RS26625 (FTO14_026625) 5397511..5398146 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FTO14_RS26630 (FTO14_026630) 5398143..5399072 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FTO14_RS26635 (FTO14_026635) 5399246..5399629 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FTO14_RS26640 (FTO14_026640) 5399629..5399871 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FTO14_RS26645 (FTO14_026645) 5400076..5400993 + 918 WP_002882812.1 alpha/beta hydrolase -
FTO14_RS26650 (FTO14_026650) 5401007..5401948 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FTO14_RS26655 (FTO14_026655) 5401993..5402430 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FTO14_RS26660 (FTO14_026660) 5402427..5403287 - 861 WP_002882807.1 virulence factor BrkB family protein -
FTO14_RS26665 (FTO14_026665) 5403281..5403880 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214787 WP_002882817.1 NZ_CP082759:c5399629-5399246 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214787 NZ_CP110235:6357-6647 [Vagococcus sp. STAA11]
ATGTTAGAAATGAAGCAGACAACCAAATTCAAAAAAGATCTGAAACGACTAAAGAAACGAAATGCCAATCTTGAAAAACT
CAAAACGGTTATGCAAATGATCGTTGAAGAAGTGCCCTTACCTGAAGAAGAATACCGTGCTCATATTTTAGAGCCGACAA
AAGATTATCTCGATTGTTGGGAGTGTCATATTTCTGGACGCAATAGCGATTGGTTATTGATTTATAAATTTTACCCTTCT
CAAAACCTTGTCTCTTTTATCCGAACCGGATCGCATTCAGATGTTTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214787 WP_004150355.1 NZ_CP082759:c5399871-5399629 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214787 NZ_CP110235:6064-6351 [Vagococcus sp. STAA11]
GTGGAAACAAAACAAAAAAAAGCGGTCATACAAGTGCGTATTGACGAAACCGTTAAAAGTCAGGCCGTAGCCGTTTTAAA
TAACCTAGGTATGGACACAAGCACCGCTATTAACGTCTTTTTCAGACAAGTAATCGCAGAAAATGGTCTGCCATTTCAAC
CAAAACAAGCAAAGTTCAATGCCGAAACCCTTGCAGCAATTAAGGAATCGGACGAAATGGTCCAAAATAGAACTGGAAAA
CGCTACACTTCGATTGACAATCTTTTTAAAGATGCTTTAGGAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References