Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4816430..4817240 Replicon chromosome
Accession NZ_CP082759
Organism Klebsiella pneumoniae strain 36

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag FTO14_RS23850 Protein ID WP_002887280.1
Coordinates 4816430..4816963 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag FTO14_RS23855 Protein ID WP_002887278.1
Coordinates 4816974..4817240 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTO14_RS23845 (FTO14_023845) 4815261..4816382 + 1122 WP_002887282.1 cupin domain-containing protein -
FTO14_RS23850 (FTO14_023850) 4816430..4816963 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
FTO14_RS23855 (FTO14_023855) 4816974..4817240 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
FTO14_RS23860 (FTO14_023860) 4817343..4818776 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
FTO14_RS23865 (FTO14_023865) 4818766..4819449 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
FTO14_RS23870 (FTO14_023870) 4819621..4821006 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
FTO14_RS23875 (FTO14_023875) 4821024..4821368 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T214784 WP_002887280.1 NZ_CP082759:c4816963-4816430 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T214784 NZ_CP110231:c2360795-2360520 [Selenomonas sputigena]
TTGAAATACAAGATCATCCCCACAGCACGTTTCCGCAAAGACGTAAAAACCGCCGCCAAACGCGGTCTGCCTATTTGCCG
TCTGGAAGAAATCGTCGACCAGCTTGCCGCTGGAACGCCATTGCCCGAAAGGAATCGCGACCATGCACTTACAGGTACAT
TCGCAGGATATCGGGAATGTCATATATTGCCAGATTGGTTACTAGTCTATCGTATTGATAACGAAATTCTCACTTTACTC
CTTCATCGAACGGGTACACACAGCGATTTGTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT214784 WP_002887278.1 NZ_CP082759:c4817240-4816974 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT214784 NZ_CP110231:c2361058-2360792 [Selenomonas sputigena]
ATGCCTTCAACCATGATACGAGTCCGCATGGATTCAGCGTTAAAAGCCCAAACGGATGCCGCACTCGCCAGTATGGGACT
CAACATGTCCGTCGCGGTCAACGCGATGGCGCGTCAGATTCTGCGCCAAGGAAGGCTTCCTTTTGAGCTTTATGCAACAA
ATCCCGAAACGATGGCCGCTCTGCAGGAGGCCGAAGAGATACGCAGAAATCCCGAGAATTTCAAATCCTATGCTTCAGCA
CATGAAATGATGGAGGATATTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References