Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 84093..85003 Replicon plasmid pKP37-vir
Accession NZ_CP082754
Organism Klebsiella pneumoniae strain 37

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag FTO11_RS27470 Protein ID WP_004026354.1
Coordinates 84093..84563 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag FTO11_RS27475 Protein ID WP_004026357.1
Coordinates 84560..85003 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTO11_RS27430 (FTO11_027425) 79409..79723 + 315 WP_011251273.1 hypothetical protein -
FTO11_RS27435 (FTO11_027430) 79732..80226 + 495 WP_011251274.1 hypothetical protein -
FTO11_RS27440 (FTO11_027435) 80232..80672 + 441 WP_011251275.1 hypothetical protein -
FTO11_RS27445 (FTO11_027440) 81097..82053 - 957 WP_011251280.1 DsbA family protein -
FTO11_RS27450 (FTO11_027445) 82113..82454 - 342 WP_011251281.1 hypothetical protein -
FTO11_RS27455 (FTO11_027450) 82468..82779 - 312 WP_011251282.1 hypothetical protein -
FTO11_RS27460 (FTO11_027455) 82796..83245 - 450 WP_011251283.1 thioredoxin fold domain-containing protein -
FTO11_RS27470 (FTO11_027465) 84093..84563 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
FTO11_RS27475 (FTO11_027470) 84560..85003 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
FTO11_RS27480 (FTO11_027475) 85104..85607 - 504 Protein_97 DUF4113 domain-containing protein -
FTO11_RS27485 (FTO11_027480) 85813..86793 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
FTO11_RS27490 (FTO11_027485) 86859..87458 + 600 Protein_99 TonB-dependent receptor -
FTO11_RS27495 (FTO11_027490) 87520..88500 + 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
FTO11_RS27500 (FTO11_027495) 88836..89489 + 654 WP_011251259.1 class I SAM-dependent methyltransferase -
FTO11_RS27505 (FTO11_027500) 89559..89825 - 267 Protein_102 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / iucA / iucB / iucC / iucD / iutA / rmpA2 1..193370 193370
- inside IScluster/Tn - iroN 77812..93751 15939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T214763 WP_004026354.1 NZ_CP082754:c84563-84093 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T214763 NZ_CP110220:c4072279-4071995 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT214763 WP_004026357.1 NZ_CP082754:c85003-84560 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT214763 NZ_CP110220:c4072511-4072269 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References