Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5398290..5398915 Replicon chromosome
Accession NZ_CP082753
Organism Klebsiella pneumoniae strain 37

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FTO11_RS26630 Protein ID WP_002882817.1
Coordinates 5398290..5398673 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FTO11_RS26635 Protein ID WP_004150355.1
Coordinates 5398673..5398915 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTO11_RS26615 (FTO11_026610) 5395656..5396558 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FTO11_RS26620 (FTO11_026615) 5396555..5397190 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FTO11_RS26625 (FTO11_026620) 5397187..5398116 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FTO11_RS26630 (FTO11_026625) 5398290..5398673 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FTO11_RS26635 (FTO11_026630) 5398673..5398915 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FTO11_RS26640 (FTO11_026635) 5399120..5400037 + 918 WP_002882812.1 alpha/beta hydrolase -
FTO11_RS26645 (FTO11_026640) 5400051..5400992 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FTO11_RS26650 (FTO11_026645) 5401037..5401474 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FTO11_RS26655 (FTO11_026650) 5401471..5402331 - 861 WP_002882807.1 virulence factor BrkB family protein -
FTO11_RS26660 (FTO11_026655) 5402325..5402924 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214760 WP_002882817.1 NZ_CP082753:c5398673-5398290 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214760 NZ_CP110220:3595829-3596086 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214760 WP_004150355.1 NZ_CP082753:c5398915-5398673 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214760 NZ_CP110220:c3596004-3595672 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References