Detailed information of TA system TA214742

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 301626..302427
• Replicon: plasmid pPM400
• Accession: NZ_CP082364
• Organism: Bacillus badius strain NBPM-293

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: A0A0C2Y625
• Locus tag: K7T73_RS21380
• Protein ID: WP_041098740.1
• Coordinates: 302017..302427 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: K7T73_RS21375
• Protein ID: WP_197072550.1
• Coordinates: 301626..302027 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7T73_RS21355 (K7T73_21315)	297012..298724	+	1713	WP_223664077.1	methyl-accepting chemotaxis protein	-
K7T73_RS21360 (K7T73_21320)	299055..299411	-	357	WP_223664078.1	helix-turn-helix transcriptional regulator	-
K7T73_RS21365 (K7T73_21325)	299710..300606	+	897	WP_052475204.1	peptide ABC transporter substrate-binding protein	-
K7T73_RS21370 (K7T73_21330)	301045..301440	+	396	WP_052475203.1	sporulation protein	-
K7T73_RS21375 (K7T73_21335)	301626..302027	+	402	WP_197072550.1	nucleotidyltransferase domain-containing protein	Antitoxin
K7T73_RS21380 (K7T73_21340)	302017..302427	+	411	WP_041098740.1	DUF86 domain-containing protein	Toxin
K7T73_RS21385 (K7T73_21345)	302490..303611	+	1122	WP_041098738.1	tyrosine recombinase XerS	-
K7T73_RS21390 (K7T73_21350)	303748..304851	-	1104	WP_041098737.1	glycerol dehydrogenase	-
K7T73_RS21395 (K7T73_21355)	305099..305662	-	564	WP_125474396.1	hypothetical protein	-
K7T73_RS21400 (K7T73_21360)	306058..307164	-	1107	WP_041098732.1	dipeptide epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..395960	395960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (27-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (8-94)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15564.96 Da
• Isoelectric Point: 6.8634

>T214742 WP_041098740.1 NZ_CP082364:302017-302427 [Bacillus badius]
MKSDVILNKIGTIERCVKRINEEYANHPDNLENYTKQDSIVLNIQRACEASIDIAMHAVAEKKLGIPQTSRDAFELLAKN
KVITEDLSFKMKAMVGFRNIAVHDYQDINLDIVKAIIEKHLQDYKDFTKQILAFQG

Nucleotide

• Length: 411 bp

>T214742 NZ_CP110201:3702456-3702731 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 15264.23 Da
• Isoelectric Point: 4.5000

>AT214742 WP_197072550.1 NZ_CP082364:301626-302027 [Bacillus badius]
IEDAEKLITDFLVDRVAPSVIYLFGSRVAGRARSNSDYDIAYLSDKHLSNYERFMLAQELAAILNRDVDLVDLKQASTVF
QTEIIETGKVLYGDHSTDKALFEMRALKNYAILNEERRVILENISERGQIYEE

Nucleotide

• Length: 402 bp

>AT214742 NZ_CP110201:c3702721-3702299 [Salmonella enterica subsp. enterica serovar Typhimurium]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A0C2Y625