Detailed information of TA system TA214740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 329203..329840
• Replicon: chromosome
• Accession: NZ_CP082363
• Organism: Bacillus badius strain NBPM-293

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A837YY90
• Locus tag: K7T73_RS01785
• Protein ID: WP_041100719.1
• Coordinates: 329490..329840 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0C2Y7Z6
• Locus tag: K7T73_RS01780
• Protein ID: WP_041100717.1
• Coordinates: 329203..329484 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7T73_RS01755 (K7T73_01755)	324432..325934	+	1503	WP_063384444.1	PH domain-containing protein	-
K7T73_RS01760 (K7T73_01760)	325897..326253	+	357	WP_223662551.1	holo-ACP synthase	-
K7T73_RS01765 (K7T73_01765)	326333..327349	+	1017	WP_041100711.1	outer membrane lipoprotein carrier protein LolA	-
K7T73_RS01770 (K7T73_01770)	327522..327728	+	207	WP_223662553.1	hypothetical protein	-
K7T73_RS01775 (K7T73_01775)	327851..329014	+	1164	WP_223662555.1	alanine racemase	-
K7T73_RS01780 (K7T73_01780)	329203..329484	+	282	WP_041100717.1	hypothetical protein	Antitoxin
K7T73_RS01785 (K7T73_01785)	329490..329840	+	351	WP_041100719.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K7T73_RS01790 (K7T73_01790)	330273..331106	+	834	WP_223662557.1	RsbT co-antagonist protein RsbRA	-
K7T73_RS01795 (K7T73_01795)	331103..331459	+	357	WP_063384450.1	STAS domain-containing protein	-
K7T73_RS01800 (K7T73_01800)	331463..331858	+	396	WP_041100725.1	anti-sigma regulatory factor	-
K7T73_RS01805 (K7T73_01805)	331870..332880	+	1011	WP_063384451.1	PP2C family protein-serine/threonine phosphatase	-
K7T73_RS01810 (K7T73_01810)	332954..333283	+	330	WP_041100729.1	anti-sigma factor antagonist	-
K7T73_RS01815 (K7T73_01815)	333283..333753	+	471	WP_063384452.1	anti-sigma B factor RsbW	-
K7T73_RS01820 (K7T73_01820)	333731..334525	+	795	WP_063384453.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12992.03 Da
• Isoelectric Point: 4.8838

>T214740 WP_041100719.1 NZ_CP082363:329490-329840 [Bacillus badius]
VIVKRGDVYFADLSPVVGSEQGGVRPVLVVQNDIGNRFSPTIIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITQLDEEMMEKVDEALQISLGLIQF

Nucleotide

• Length: 351 bp

>T214740 NZ_CP110201:2446354-2446638 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10674.25 Da
• Isoelectric Point: 8.9070

>AT214740 WP_041100717.1 NZ_CP082363:329203-329484 [Bacillus badius]
VSESSATTEVLIRLPQQFVKELDGYAELEKVNRSEFIYRATKMYLRERKKRQIRESMRRGYMEMAKINLAIASEALQAEC
EAGNTVERLVSGG

Nucleotide

• Length: 282 bp

>AT214740 NZ_CP110201:2446116-2446364 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A837YY90

Antitoxin

• AlphaFold DB: A0A0C2Y7Z6