Detailed information of TA system TA021474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2207774..2208288
• Replicon: chromosome
• Accession: NC_007793
• Organism: Staphylococcus aureus subsp. aureus USA300 FPR3757

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SAUSA300_RS11235
• Protein ID: WP_000581792.1
• Coordinates: 2207774..2207983 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: SAUSA300_RS11240
• Protein ID: WP_000138932.1
• Coordinates: 2207995..2208288 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAUSA300_RS11215	2203282..2204640	-	1359	WP_000611462.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SAUSA300_RS11220	2204655..2205725	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
SAUSA300_RS11225	2206043..2207245	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SAUSA300_RS11230	2207509..2207646	-	138	WP_000828354.1	hypothetical protein	-
SAUSA300_RS11235	2207774..2207983	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SAUSA300_RS11240	2207995..2208288	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAUSA300_RS11245	2208454..2209938	+	1485	WP_000571549.1	cardiolipin synthase	-
SAUSA300_RS11250	2209966..2210613	+	648	WP_001187629.1	HD domain-containing protein	-
SAUSA300_RS11265	2211426..2212298	-	873	WP_000725802.1	membrane protein insertase YidC	-
SAUSA300_RS11270	2212384..2213025	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T21474 WP_000581792.1 NC_007793:c2207983-2207774 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T21474 NC_007793:c2207983-2207774 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT21474 WP_000138932.1 NC_007793:c2208288-2207995 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT21474 NC_007793:c2208288-2207995 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13