Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20078..20721 Replicon plasmid pVCSW7
Accession NZ_CP082358
Organism Escherichia coli strain VCSW7

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag K7X31_RS22445 Protein ID WP_001044768.1
Coordinates 20078..20494 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag K7X31_RS22450 Protein ID WP_001261287.1
Coordinates 20491..20721 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X31_RS22435 (15929) 15929..17362 - 1434 WP_105633033.1 SH3 domain-containing C40 family peptidase -
K7X31_RS22985 (17452) 17452..17614 - 163 Protein_14 transcriptional regulator -
K7X31_RS22440 (18482) 18482..19916 + 1435 Protein_15 AAA family ATPase -
K7X31_RS22445 (20078) 20078..20494 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
K7X31_RS22450 (20491) 20491..20721 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K7X31_RS22455 (21286) 21286..21699 + 414 WP_000465043.1 hypothetical protein -
K7X31_RS22460 (21701) 21701..22483 + 783 WP_105633036.1 site-specific integrase -
K7X31_RS22465 (22656) 22656..23009 + 354 WP_000864812.1 colicin M immunity protein -
K7X31_RS22470 (23059) 23059..23874 - 816 WP_000449473.1 lipid II-degrading bacteriocin colicin M -
K7X31_RS22475 (24117) 24117..24644 + 528 WP_000203268.1 colicin B immunity protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - faeC / faeD / faeE / faeF / faeH / faeI / faeJ 1..89947 89947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T214719 WP_001044768.1 NZ_CP082358:c20494-20078 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T214719 NZ_CP110198:316725-317093 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT214719 WP_001261287.1 NZ_CP082358:c20721-20491 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT214719 NZ_CP110198:316507-316728 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References