Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4262354..4262851 Replicon chromosome
Accession NZ_CP082357
Organism Escherichia coli strain VCSW7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K7X31_RS20225 Protein ID WP_001173343.1
Coordinates 4262354..4262527 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K7X31_RS20230 Protein ID WP_000398619.1
Coordinates 4262555..4262851 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X31_RS20215 (4259024) 4259024..4260481 + 1458 WP_000856819.1 dipeptide/tripeptide permease DtpC -
K7X31_RS20220 (4260718) 4260718..4262235 + 1518 WP_223024764.1 lysine--tRNA ligase -
K7X31_RS20225 (4262354) 4262354..4262527 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K7X31_RS20230 (4262555) 4262555..4262851 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K7X31_RS20235 (4263080) 4263080..4263352 - 273 WP_024704987.1 GNAT family N-acetyltransferase -
K7X31_RS20240 (4263364) 4263364..4263594 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
K7X31_RS20245 (4263775) 4263775..4265406 + 1632 WP_001216477.1 sensor histidine kinase -
K7X31_RS20250 (4265403) 4265403..4266122 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T214717 WP_001173343.1 NZ_CP082357:c4262527-4262354 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T214717 NZ_CP110196:37431-37709 [Klebsiella pneumoniae]
ATGGAGATATTCTGGACCATACTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTATATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT214717 WP_000398619.1 NZ_CP082357:c4262851-4262555 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT214717 NZ_CP110196:37162-37431 [Klebsiella pneumoniae]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGATATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATAAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References