Detailed information of TA system TA214716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4163030..4163625
• Replicon: chromosome
• Accession: NZ_CP082357
• Organism: Escherichia coli strain VCSW7

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: K7X31_RS19705
• Protein ID: WP_000239581.1
• Coordinates: 4163030..4163380 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: K7X31_RS19710
• Protein ID: WP_001223213.1
• Coordinates: 4163374..4163625 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X31_RS19685 (4158476)	4158476..4159498	-	1023	WP_024180251.1	ABC transporter permease	-
K7X31_RS19690 (4159512)	4159512..4161014	-	1503	WP_223024757.1	sugar ABC transporter ATP-binding protein	-
K7X31_RS19695 (4161155)	4161155..4162111	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
K7X31_RS19700 (4162421)	4162421..4162951	+	531	WP_000055075.1	inorganic diphosphatase	-
K7X31_RS19705 (4163030)	4163030..4163380	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
K7X31_RS19710 (4163374)	4163374..4163625	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
K7X31_RS19715 (4163837)	4163837..4164178	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
K7X31_RS19720 (4164181)	4164181..4167960	-	3780	WP_000060886.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T214716 WP_000239581.1 NZ_CP082357:c4163380-4163030 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T214716 NZ_CP110195:41456-41761 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT214716 WP_001223213.1 NZ_CP082357:c4163625-4163374 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT214716 NZ_CP110195:41236-41454 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7