Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 884361..885015 Replicon chromosome
Accession NZ_CP082357
Organism Escherichia coli strain VCSW7

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K7X31_RS04205 Protein ID WP_000244777.1
Coordinates 884608..885015 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K7X31_RS04200 Protein ID WP_000354046.1
Coordinates 884361..884627 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X31_RS04180 (880449) 880449..881882 - 1434 WP_024210543.1 6-phospho-beta-glucosidase BglA -
K7X31_RS04185 (881927) 881927..882238 + 312 WP_001604811.1 N(4)-acetylcytidine aminohydrolase -
K7X31_RS04190 (882402) 882402..883061 + 660 WP_000250269.1 hemolysin III family protein -
K7X31_RS04195 (883138) 883138..884118 - 981 WP_223024930.1 tRNA-modifying protein YgfZ -
K7X31_RS04200 (884361) 884361..884627 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K7X31_RS04205 (884608) 884608..885015 + 408 WP_000244777.1 protein YgfX Toxin
K7X31_RS04210 (885055) 885055..885576 - 522 WP_001055872.1 flavodoxin FldB -
K7X31_RS04215 (885688) 885688..886584 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
K7X31_RS04220 (886609) 886609..887319 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K7X31_RS04225 (887325) 887325..889058 + 1734 WP_223024931.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T214696 WP_000244777.1 NZ_CP082357:884608-885015 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214696 NZ_CP110190:c5545672-5545382 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214696 WP_000354046.1 NZ_CP082357:884361-884627 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214696 NZ_CP110190:c5545968-5545669 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References