Detailed information of TA system TA214694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 290342..290973
• Replicon: chromosome
• Accession: NZ_CP082357
• Organism: Escherichia coli strain VCSW7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K7X31_RS01245
• Protein ID: WP_223024845.1
• Coordinates: 290342..290617 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: K7X31_RS01250
• Protein ID: WP_000593555.1
• Coordinates: 290614..290973 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X31_RS01230 (285346)	285346..286413	+	1068	WP_000361507.1	HlyD family efflux transporter periplasmic adaptor subunit	-
K7X31_RS01235 (286410)	286410..289145	+	2736	WP_223024844.1	ribosome-associated ATPase/putative transporter RbbA	-
K7X31_RS01240 (289145)	289145..290269	+	1125	WP_001216257.1	ABC transporter permease	-
K7X31_RS01245 (290342)	290342..290617	+	276	WP_223024845.1	type II toxin-antitoxin system HicA family toxin	Toxin
K7X31_RS01250 (290614)	290614..290973	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K7X31_RS01255 (291093)	291093..291494	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
K7X31_RS01260 (291499)	291499..292305	-	807	WP_000173690.1	nickel import ATP-binding protein NikE	-
K7X31_RS01265 (292302)	292302..293066	-	765	WP_217800989.1	nickel import ATP-binding protein NikD	-
K7X31_RS01270 (293066)	293066..293899	-	834	WP_001008967.1	nickel ABC transporter permease subunit NikC	-
K7X31_RS01275 (293896)	293896..294840	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10127.83 Da
• Isoelectric Point: 10.7822

>T214694 WP_223024845.1 NZ_CP082357:290342-290617 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RGWLLSIGVKP

Nucleotide

• Length: 276 bp

>T214694 NZ_CP110190:c5454141-5453851 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT214694 WP_000593555.1 NZ_CP082357:290614-290973 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT214694 NZ_CP110190:c5454437-5454138 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6