Detailed information of TA system TA214688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4198765..4199360
• Replicon: chromosome
• Accession: NZ_CP082355
• Organism: Escherichia coli strain VCSW5

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: K7X33_RS20000
• Protein ID: WP_000239581.1
• Coordinates: 4198765..4199115 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: K7X33_RS20005
• Protein ID: WP_001223213.1
• Coordinates: 4199109..4199360 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X33_RS19980 (4194219)	4194219..4195241	-	1023	WP_024180251.1	ABC transporter permease	-
K7X33_RS19985 (4195255)	4195255..4196757	-	1503	WP_001095659.1	sugar ABC transporter ATP-binding protein	-
K7X33_RS19990 (4196890)	4196890..4197846	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
K7X33_RS19995 (4198156)	4198156..4198686	+	531	WP_000055075.1	inorganic diphosphatase	-
K7X33_RS20000 (4198765)	4198765..4199115	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
K7X33_RS20005 (4199109)	4199109..4199360	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
K7X33_RS20010 (4199572)	4199572..4199913	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
K7X33_RS20015 (4199916)	4199916..4203695	-	3780	WP_000060886.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T214688 WP_000239581.1 NZ_CP082355:c4199115-4198765 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T214688 NZ_CP110189:c1053727-1053440 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT214688 WP_001223213.1 NZ_CP082355:c4199360-4199109 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT214688 NZ_CP110189:c1053968-1053717 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7