Detailed information of TA system TA214684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3527492..3528329
• Replicon: chromosome
• Accession: NZ_CP082355
• Organism: Escherichia coli strain VCSW5

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: K7X33_RS16870
• Protein ID: WP_000227790.1
• Coordinates: 3527787..3528329 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: K7X33_RS16865
• Protein ID: WP_001297137.1
• Coordinates: 3527492..3527803 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X33_RS16840 (3522512)	3522512..3523459	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
K7X33_RS16845 (3523481)	3523481..3525472	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
K7X33_RS16850 (3525462)	3525462..3526076	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
K7X33_RS16855 (3526076)	3526076..3526405	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
K7X33_RS16860 (3526417)	3526417..3527307	+	891	WP_000971339.1	heme o synthase	-
K7X33_RS16865 (3527492)	3527492..3527803	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
K7X33_RS16870 (3527787)	3527787..3528329	+	543	WP_000227790.1	GNAT family N-acetyltransferase	Toxin
K7X33_RS16875 (3528385)	3528385..3529320	-	936	WP_024180261.1	tetratricopeptide repeat protein	-
K7X33_RS16880 (3529727)	3529727..3531091	+	1365	WP_001000955.1	MFS transporter	-
K7X33_RS16885 (3531219)	3531219..3531710	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
K7X33_RS16890 (3531878)	3531878..3532789	+	912	WP_000705836.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19790.95 Da
• Isoelectric Point: 8.0335

>T214684 WP_000227790.1 NZ_CP082355:3527787-3528329 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLNKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T214684 NZ_CP110189:c1024103-1023570 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT214684 WP_001297137.1 NZ_CP082355:3527492-3527803 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT214684 NZ_CP110189:c1024369-1024100 [Vibrio cholerae]
ATGGCAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTT
AAAAAGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGG
TTAAAGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTT
AAATTTACAGAGAAGAGCGGTATCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3