Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3493415..3494033 Replicon chromosome
Accession NZ_CP082355
Organism Escherichia coli strain VCSW5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K7X33_RS16700 Protein ID WP_001291435.1
Coordinates 3493815..3494033 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K7X33_RS16695 Protein ID WP_000344800.1
Coordinates 3493415..3493789 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X33_RS16685 (3488504) 3488504..3489697 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K7X33_RS16690 (3489720) 3489720..3492869 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K7X33_RS16695 (3493415) 3493415..3493789 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K7X33_RS16700 (3493815) 3493815..3494033 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K7X33_RS16705 (3494205) 3494205..3494756 + 552 WP_000102564.1 maltose O-acetyltransferase -
K7X33_RS16710 (3494872) 3494872..3495342 + 471 WP_000136192.1 YlaC family protein -
K7X33_RS16715 (3495506) 3495506..3497056 + 1551 WP_001305493.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K7X33_RS16720 (3497098) 3497098..3497451 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K7X33_RS16730 (3497830) 3497830..3498141 + 312 WP_000409911.1 MGMT family protein -
K7X33_RS16735 (3498172) 3498172..3498744 - 573 WP_000779820.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214683 WP_001291435.1 NZ_CP082355:3493815-3494033 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T214683 NZ_CP110189:c1023123-1022821 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT214683 WP_000344800.1 NZ_CP082355:3493415-3493789 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT214683 NZ_CP110189:c1023368-1023111 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References