Detailed information of TA system TA021468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1986965..1987741
• Replicon: chromosome
• Accession: NC_007793
• Organism: Staphylococcus aureus subsp. aureus USA300 FPR3757

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: SAUSA300_RS09870
• Protein ID: WP_000031108.1
• Coordinates: 1986965..1987117 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: SAUSA300_RS09875
• Protein ID: WP_001251224.1
• Coordinates: 1987142..1987741 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAUSA300_RS09850	1982928..1983749	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
SAUSA300_RS09855	1984212..1985597	-	1386	WP_000116224.1	class II fumarate hydratase	-
SAUSA300_RS09860	1985793..1986188	-	396	WP_000901021.1	hypothetical protein	-
SAUSA300_RS09870	1986965..1987117	-	153	WP_000031108.1	hypothetical protein	Toxin
SAUSA300_RS09875	1987142..1987741	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
SAUSA300_RS09880	1987900..1988370	-	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
SAUSA300_RS09885	1988375..1989502	-	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
SAUSA300_RS09890	1989653..1990375	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
SAUSA300_RS09895	1990368..1991825	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T21468 WP_000031108.1 NC_007793:c1987117-1986965 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T21468 NC_007793:c1987117-1986965 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT21468 WP_001251224.1 NC_007793:c1987741-1987142 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT21468 NC_007793:c1987741-1987142 [Staphylococcus aureus subsp. aureus USA300_FPR3757]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4