Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 882891..883545 Replicon chromosome
Accession NZ_CP082355
Organism Escherichia coli strain VCSW5

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K7X33_RS04215 Protein ID WP_000244777.1
Coordinates 883138..883545 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K7X33_RS04210 Protein ID WP_000354046.1
Coordinates 882891..883157 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X33_RS04190 (878979) 878979..880412 - 1434 WP_024210543.1 6-phospho-beta-glucosidase BglA -
K7X33_RS04195 (880457) 880457..880768 + 312 WP_001604811.1 N(4)-acetylcytidine aminohydrolase -
K7X33_RS04200 (880932) 880932..881591 + 660 WP_000250269.1 hemolysin III family protein -
K7X33_RS04205 (881668) 881668..882648 - 981 WP_223024930.1 tRNA-modifying protein YgfZ -
K7X33_RS04210 (882891) 882891..883157 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K7X33_RS04215 (883138) 883138..883545 + 408 WP_000244777.1 protein YgfX Toxin
K7X33_RS04220 (883585) 883585..884106 - 522 WP_001055872.1 flavodoxin FldB -
K7X33_RS04225 (884218) 884218..885114 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
K7X33_RS04230 (885139) 885139..885849 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K7X33_RS04235 (885855) 885855..887588 + 1734 WP_223024931.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T214671 WP_000244777.1 NZ_CP082355:883138-883545 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214671 NZ_CP110170:1957090-1957192 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214671 WP_000354046.1 NZ_CP082355:882891-883157 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214671 NZ_CP110170:c1957199-1957054 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References